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Updated: May 11, 2026

Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets
11:19

Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets

Published on: July 7, 2010

Histone demethylase RBP2 promotes lung tumorigenesis and cancer metastasis

Yu-Ching Teng1, Cheng-Feng Lee, Ying-Shiuan Li

  • 1Genomics Research Center, Academia Sinica, 128, Academia Rd., Sec. 2, Nankang, Taipei, 115, Taiwan.

Cancer Research
|June 1, 2013
PubMed

Insights

Retinoblastoma binding protein RBP2 (KDM5A) drives lung cancer progression by promoting cell proliferation and metastasis. Its depletion suppresses tumor growth, revealing RBP2 as a potential therapeutic target for lung cancer treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Epigenetics

Background:

  • Retinoblastoma binding protein RBP2 (KDM5A) is a histone demethylase implicated in gastric cancer and drug-resistant lung cancer.
  • Previous studies in mice suggest RBP2 ablation suppresses tumor initiation, but its role in human lung cancer requires further definition.

Purpose of the Study:

  • To investigate the oncogenic role of RBP2 in human lung cancer.
  • To elucidate the mechanisms by which RBP2 promotes lung tumorigenesis and progression.

Main Methods:

  • Analysis of RBP2 expression in human lung cancer tissues.
  • Assessment of RBP2 depletion effects on cancer cell proliferation, motility, invasion, and metastasis.
  • Investigation of RBP2's demethylase and DNA-binding activities.
  • Gene expression analysis (microarray) and promoter binding assays.
  • Rescue experiments with target gene overexpression.

Main Results:

  • RBP2 is overexpressed in human lung cancer, and its depletion inhibits proliferation, motility, invasion, and metastasis.
  • RBP2 oncogenicity depends on its demethylase and DNA-binding activities.
  • RBP2 upregulates cyclins D1/E1 and ITGB1 while downregulating p27 (CDKN1B), directly binding to their promoters.
  • Overexpression of cyclin D1, cyclin E1, or ITGB1 rescues RBP2-mediated proliferation or invasion.

Conclusions:

  • RBP2 plays a significant oncogenic role in lung cancer initiation and progression.
  • RBP2 targets include cyclins D1/E1, p27, and ITGB1, mediating its effects on proliferation and metastasis.
  • RBP2 represents a potential therapeutic target for lung cancer.

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