Expression, purification, crystallization and preliminary X-ray crystallographic study of the nucleocapsid protein of
Keisuke Komoda1, Masanori Narita, Isao Tanaka
1Graduate School of Life Sciences, Hokkaido University, Sapporo 060-0810, Japan.
Abstract:
Tomato spotted wilt virus (TSWV), which causes severe damage to various agricultural crops such as tomato, pepper, lettuce and peanut, is a negative-stranded RNA virus belonging to the Tospovirus genus of the Bunyaviridae family. Viral genomic RNA molecules are packaged in the form of ribonucleoprotein complexes, each of which contains one viral RNA molecule that is coated with many nucleocapsid (N) proteins. Here, the expression and crystallization of TSWV N protein are reported. Native and selenomethionine-substituted crystals of N protein belonged to the same space group P2(1). Their unit-cell parameters were a = 66.8, b = 97.2, c = 72.0 Å, β = 112.8° and a = 66.5, b = 96.3, c = 72.1 Å, β = 113.4°, respectively.
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