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Mass Spectrometric Approaches to Study Protein Structure and Interactions in Lyophilized Powders
Published on: April 14, 2015
Structure and dynamics in proteins of pharmacological interest
1Institut für Molekularbiologie und Biophysik, Eidgenössische Technische Hochschule-Hönggerberg, Zürich, Switzerland.
Abstract:
This paper begins with a brief survey of the standard nuclear magnetic resonance (NMR) method for protein structure determination in solution, which has been applied successfully with numerous globular proteins with molecular weights in the range from 3,000 to 15,000. The results obtained show that for the core of globular proteins, the quality of the structures determined in solution can be comparable to that achieved with diffraction techniques using protein single crystals. In addition, they also indicate that a complete description of proteins in solution may include short-lived, transient structural features that could be of crucial importance for the functional properties. Several supplementary NMR techniques capable of characterizing diverse aspects of flexible polypeptide chains are discussed.
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