Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Image analysis for measuring the rDNA transcriptional activity].

L Q Chen1, Q H He, G H Wang

  • 1Biological Genetics Department, Beijing Medical University.

Yi Chuan Xue Bao = Acta Genetica Sinica
|January 1, 1990
PubMed
Summary

Low doses of AMD inhibit silver stainability of NORs, reflecting rDNA transcriptional activity. Ag-Nucleoli size serves as a precise indicator for measuring rDNA transcription via silver staining.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Taking stroke prevention and treatment as a breakthrough point to establish a new grid-based system for chronic disease prevention and treatment].

Zhonghua yi xue za zhi·2025
Same author

[Immunotherapy: progress and challenges of a revolutionary treatment for gastric cancer].

Zhonghua wai ke za zhi [Chinese journal of surgery]·2025
Same author

[The impact of neoadjuvant immunotherapy on postoperative complications and short-term efficacy in patients with advanced gastric cancer].

Zhonghua wai ke za zhi [Chinese journal of surgery]·2025
Same author

[Attach importance to functional evaluation after gastric-preserving surgery].

Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery·2025
Same author

[Rethinking the scope of lymphadenectomy after neoadjuvant immunotherapy for locally advanced gastric cancer].

Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery·2024
Same author

[The Development of SpCas9 Variants with High Specificity and Efficiency Based on the HH Theory].

Molekuliarnaia biologiia·2024

Area of Science:

  • Molecular Biology
  • Cytogenetics

Context:

  • Nucleolar Organizer Regions (NORs) are crucial for ribosome biogenesis.
  • Silver staining is a common method to visualize NORs and assess their activity.
  • Actinomycin D (AMD) is known to affect cellular processes, including transcription.

Purpose:

  • To investigate the correlation between Actinomycin D (AMD) concentration and the silver stainability of NORs.
  • To determine if AMD concentration can serve as a reliable indicator of rDNA transcriptional activity.
  • To evaluate the utility of Ag-Nucleoli size as a quantitative measure of rDNA transcription.

Summary:

  • This study demonstrated that low doses of Actinomycin D (AMD) inhibit the silver stainability of Nucleolar Organizer Regions (NORs) during interphase and metaphase.

Related Experiment Videos

  • Using the HABM image analysis system, researchers measured variations in NOR silver stainability across different AMD concentrations.
  • Data analysis revealed a significant variability in Ag-Nucleoli and a positive correlation between Ag-Nucleoli size and AMD concentration.

    • Impact:

    • The findings suggest that AMD concentration directly reflects rDNA transcriptional activity in NORs.
    • Ag-Nucleoli size, as measured by silver staining, is proposed as a precise indicator for assessing rDNA transcriptional activity.
    • This research provides a refined method for quantifying gene transcription related to ribosome synthesis.