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Rapid Detection of Fecal Antigen of Helicobacter pylori Infection Based on Double Antibody Sandwich Detection Technology
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Stool antigen tests for the detection of Helicobacter pylori in children

Abolfazl Iranikhah1, Mohammad-Reza Ghadir, Saeed Sarkeshikian

  • 1Department of Pediatric Gastroenterology, Qom University of Medical Sciences, Qom, Iran.

Iranian Journal of Pediatrics
|June 1, 2013
PubMed

Insights

The stool antigen test is a reliable, non-invasive method for detecting Helicobacter pylori (H. pylori) infection in children with abdominal pain. This rapid diagnostic technique shows high sensitivity and specificity, potentially replacing invasive endoscopy.

Area of Science:

  • Pediatric Gastroenterology
  • Infectious Diseases
  • Diagnostic Accuracy Studies

Background:

  • Reliable non-invasive methods are needed for childhood Helicobacter pylori (H. pylori) detection.
  • Stool antigen tests offer a non-invasive alternative, but their accuracy in developing countries requires evaluation.
  • This study focuses on H. pylori diagnosis in Iranian children with recurrent abdominal pain.

Purpose of the Study:

  • To evaluate the performance of the H. pylori stool antigen (HpSA) test in Iranian children.
  • To determine the accuracy of HpSA compared to endoscopy and biopsy.
  • To assess the utility of HpSA as a diagnostic tool for H. pylori infection in pediatric patients.

Main Methods:

  • 103 children with recurrent abdominal pain underwent endoscopy and biopsy.
  • H. pylori stool antigen (HpSA) tests were performed using enzyme-linked immunosorbent assay.
  • Sensitivity, specificity, and likelihood ratios of HpSA were calculated against gastric biopsy cultures.

Main Results:

  • H. pylori infection was detected in 39.8% by biopsy and 37.8% by HpSA.
  • The HpSA test demonstrated a sensitivity of 85% and specificity of 93%.
  • Positive and negative likelihood ratios for HpSA were 89.7% and 90%, respectively.

Conclusions:

  • The H. pylori stool antigen test is a sensitive and specific, low-cost diagnostic method for children.
  • HpSA can effectively replace endoscopy and biopsy for H. pylori detection in this pediatric population.
  • Results are comparable to international findings, supporting HpSA's use in clinical settings.
Abstract

Keywords:
ChildrenHelicobacter PyloriStool Antigen Tests

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