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Immunoprecipitation

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
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Updated: May 10, 2026

High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis

High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis

Published on: October 15, 2019

Immune complexome analysis.

Kaname Ohyama¹, Naotaka Kuroda

¹Course of Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan.

Advances in Clinical Chemistry

|June 4, 2013

Summary

This summary is machine-generated.

Identifying antigens in immune complexes (ICs) offers insights into diseases. Mass spectrometry-based immunoproteomics and immune complexome analysis provide comprehensive antigen profiling for improved diagnostics and therapeutics.

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Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation

Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation

Published on: August 21, 2019

Area of Science:

Immunology
Proteomics
Biochemistry

Background:

Immune complexes (ICs) are crucial indicators of ongoing immune responses.
Antigen identification within ICs is vital for understanding autoimmune diseases, infections, cancer, and transplantation outcomes.
Profiling antigens in ICs offers more relevant information than free antigens and surpasses traditional immunoblotting methods.

Purpose of the Study:

To introduce and detail mass spectrometry (MS)-based methodologies for comprehensive antigen identification in ICs.
To highlight the utility of immunoproteomics and immune complexome analysis for disease-associated antigen discovery.

Main Methods:

Immunoproteomics: Combines protein separation (2D electrophoresis, gel-free), immunological detection (Western blotting), and MS, or immunocapture with MS.
Immune Complexome Analysis: Involves IC separation from serum, followed by direct tryptic digestion and nano-liquid chromatography-tandem MS (LC-MS/MS).

Main Results:

These MS-based approaches enable comprehensive identification and profiling of multiple antigens within ICs.
The methods provide a detailed understanding of the antigenic composition of ICs, aiding in disease biomarker discovery.

Conclusions:

Mass spectrometry-based immunoproteomics and immune complexome analysis are powerful tools for in-depth antigen identification in ICs.
These advanced techniques hold significant potential for developing novel diagnostic and therapeutic strategies for various diseases.