Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Defenses Against Pathogens and Herbivores

Defenses Against Pathogens and Herbivores

Plants present a rich source of nutrients for many organisms, making it a target for herbivores and infectious agents. Plants, though lacking a proper immune system, have developed an array of constitutive and inducible defenses to fend off these attacks.

Plant Breeding and Biotechnology

Plant Breeding and Biotechnology

Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.

Transgenic Plants

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Engineering an Exo70 integrated domain of a barley NLR for improved blast resistance.

The Plant cell·2026

Same author

Molecular mimicry of a pathogen virulence target by a plant immune receptor.

Science (New York, N.Y.)·2026

Same author

Haplotype-phased genomes of the barley leaf rust pathogen reveal evidence of repeat element expansion and somatic hybridization.

G3 (Bethesda, Md.)·2026

Same author

Elucidating the ancestral role of Class I HD-Zip transcription factors in land plants.

Plant physiology·2026

Same author

Beyond nitrogen: phosphate controls root nodule symbiosis commitment.

Trends in plant science·2026

Same author

Haplotype-Resolved Genomes of Diverse Oat Crown Rust Isolates Reveal Global Dispersion of Long-Lived Clonal Haplotypes with Limited Recombination.

Molecular plant-microbe interactions : MPMI·2026

Search research articles

Related Experiment Video

Updated: May 10, 2026

Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem

Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem

Published on: October 1, 2015

Engineering plant disease resistance based on TAL effectors.

Sebastian Schornack¹, Matthew J Moscou, Eric R Ward

¹Sainsbury Laboratory, University of Cambridge, Cambridge, CB2 1LR, United Kingdom.

Annual Review of Phytopathology

|June 4, 2013

Summary

Transcription activator-like (TAL) effectors from bacteria target plant genes. Their DNA-binding code allows prediction and design for genome editing, offering new disease control strategies.

More Related Videos

High-Throughput Identification of Resistance to Pseudomonas syringae pv. Tomato in Tomato using Seedling Flood Assay

High-Throughput Identification of Resistance to Pseudomonas syringae pv. Tomato in Tomato using Seedling Flood Assay

Published on: March 10, 2020

Split Green Fluorescent Protein System to Visualize Effectors Delivered from Bacteria During Infection

Split Green Fluorescent Protein System to Visualize Effectors Delivered from Bacteria During Infection

Published on: May 24, 2018

Related Experiment Videos

Last Updated: May 10, 2026

Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem

Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem

Published on: October 1, 2015

High-Throughput Identification of Resistance to Pseudomonas syringae pv. Tomato in Tomato using Seedling Flood Assay

High-Throughput Identification of Resistance to Pseudomonas syringae pv. Tomato in Tomato using Seedling Flood Assay

Published on: March 10, 2020

Split Green Fluorescent Protein System to Visualize Effectors Delivered from Bacteria During Infection

Split Green Fluorescent Protein System to Visualize Effectors Delivered from Bacteria During Infection

Published on: May 24, 2018

Area of Science:

Molecular Biology
Plant Pathology
Genetics

Background:

Transcription activator-like (TAL) effectors are proteins secreted by plant-pathogenic bacteria.
These effectors manipulate host plant gene expression to facilitate infection.

Purpose of the Study:

To elucidate the DNA-binding mechanism of TAL effectors.
To explore the application of TAL effector DNA-binding code for genome manipulation.
To leverage plant resistance mechanisms against TAL effectors for disease control.

Main Methods:

Analysis of the TAL effector DNA-binding code.
Design of synthetic DNA-binding domains based on the TAL effector code.
Investigation of plant resistance strategies.

Main Results:

TAL effectors bind DNA via a specific, one-to-one correspondence between amino acid residues and DNA bases.
This code enables accurate prediction of natural TAL effector binding sites.
Novel synthetic DNA-binding domains can be designed for targeted applications.
Plant resistance mechanisms provide insights into novel disease control strategies.

Conclusions:

The TAL effector DNA-binding code is a powerful tool for understanding and engineering gene regulation.
This knowledge facilitates targeted genome manipulation and the development of resistance strategies against plant pathogens.