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Related Experiment Video

Updated: Apr 28, 2026

Generation of Aligned Functional Myocardial Tissue Through Microcontact Printing

Generation of Aligned Functional Myocardial Tissue Through Microcontact Printing

Published on: March 19, 2013

Sorting inactivated cells using cell-imprinted polymer thin films.

Kangning Ren¹, Niaz Banaei, Richard N Zare

¹Department of Chemistry, Stanford University, Stanford, California 94305-5080, USA.

|June 4, 2013

Summary

This summary is machine-generated.

Inactivating cells with aldehydes like formaldehyde or glutaraldehyde significantly improves artificial receptor selectivity for cell capture. This method enhances capture efficiency and reduces biohazard risks when analyzing cells, including Mycobacterium tuberculosis.

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Last Updated: Apr 28, 2026

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Published on: March 18, 2021

Area of Science:

Biomaterials Science
Surface Chemistry
Microbiology

Background:

Cell imprinting in poly(dimethylsiloxane) films creates artificial receptors for cell capture via size, shape, and chemical recognition.
Previous methods relied on inherent cell properties for capture, which could be affected by cell surface variations and excreted substances.

Purpose of the Study:

To investigate the effect of cell inactivation using aldehydes on the selectivity and efficiency of cell imprinting for artificial receptor development.
To explore the mechanisms behind enhanced cell capture following inactivation.
To assess the potential of this method for handling hazardous biological samples like Mycobacterium tuberculosis.

Main Methods:

Poly(dimethylsiloxane) films were used to create artificial cell receptors.
Cells (including Mycobacterium tuberculosis strains) were inactivated using formaldehyde (4%), glutaraldehyde (2%), or a combination.
Cell capture selectivity and efficiency were compared between living and inactivated cells.

Main Results:

Cell inactivation with aldehydes improved capture selectivity by a factor of 3 compared to living cells.
Enhanced capture efficiency was attributed to cell surface rigidification and elimination of interfering excreted chemicals.
The method demonstrated effectiveness with different strains of Mycobacterium tuberculosis.

Conclusions:

Inactivating cells with aldehydes markedly improves the selectivity of artificial cell receptors created by cell imprinting.
This approach offers a more robust and safer method for cell capture, particularly for virulent microorganisms.
The findings have implications for developing improved diagnostic and analytical tools in microbiology and beyond.