Electroenhanced solid-phase microextraction of methamphetamine with commercial fibers.

Area of Science:

• Analytical Chemistry
• Forensic Science
• Biomedical Analysis

Background:

• Conventional solid-phase microextraction (SPME) methods for methamphetamine analysis in urine often require complex sample preparation steps.
• Developing efficient and sensitive extraction techniques is crucial for accurate methamphetamine determination.

Purpose of the Study:

• To investigate and optimize an electroenhanced solid-phase microextraction (EE-SPME) method for methamphetamine determination in urine samples.
• To compare the efficiency of EE-SPME with conventional SPME for methamphetamine extraction.

Main Methods:

• Utilized commercial SPME fibers in direct immersion mode with an applied potential for methamphetamine extraction.
• Optimized experimental conditions including applied potential, sample pH, extraction, and desorption times.
• Analyzed extracted methamphetamine using gas chromatographic mass spectrometric (GC-MS) detection.

Main Results:

• EE-SPME demonstrated significantly higher extraction efficiency, achieving a 159-fold enrichment factor compared to conventional SPME.
• The method simplified sample preparation by eliminating the need for alkalization and derivatization.
• Achieved a linear calibration range of 0.5-15 ng/mL (r=0.9948) with a limit of detection of 0.25 ng/mL in human urine.
• Demonstrated satisfactory relative standard deviation of 6.12% (n=3) for methamphetamine analysis in urine samples.

Conclusions:

• EE-SPME is a highly effective and simplified technique for the quantitative determination of methamphetamine in human urine.
• The method offers improved sensitivity and efficiency, making it a valuable tool for forensic and clinical analysis.