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Related Experiment Video

Updated: May 10, 2026

A Standardized Liquid Biopsy Preanalytical Protocol for Downstream Circulating-Free DNA Applications
05:26

A Standardized Liquid Biopsy Preanalytical Protocol for Downstream Circulating-Free DNA Applications

Published on: September 16, 2022

Circulating cell free DNA: Preanalytical considerations.

Safia El Messaoudi1, Fanny Rolet, Florent Mouliere

  • 1IRCM, Institut de Recherche en Cancérologie de Montpellier, Montpellier, F-34298, France; INSERM, U896, Montpellier, F-34298, France; Université Montpellier1, Montpellier, F-34298, France; Institut régional du Cancer Montpellier, Montpellier, F-34298, France.

Clinica Chimica Acta; International Journal of Clinical Chemistry
|June 4, 2013
PubMed
Summary
This summary is machine-generated.

Standardizing sample handling for cell-free DNA (ccfDNA) analysis is crucial for clinical practice. This review identifies optimal preanalytical protocols to ensure reliable ccfDNA testing in oncology and prenatal diagnostics.

Keywords:
BRAFCirculating cell-free DNADIIDNA integrity indexDiagnosisEDTAKRASPreanalytical handlingQ-PCRRTStabilityStandard operating procedureccfDNAcirculating cell-free DNAethylenediaminetetraacetic acidquantitative polymerase chain reactionroom temperaturev-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog [GenBank: NG_007524.1]v-raf murine sarcoma viral oncogene homolog B1 [GenBank: NG_007873.2]

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Last Updated: May 10, 2026

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Clinical Diagnostics

Background:

  • Circulating cell-free DNA (ccfDNA) analysis shows promise in oncology and prenatal diagnosis.
  • Lack of standardized sample handling protocols hinders clinical translation of ccfDNA analysis.
  • Existing reviews highlight the need for consistent preanalytical procedures.

Purpose of the Study:

  • To review preanalytical factors impacting ccfDNA concentration and fragmentation.
  • To identify optimal preanalytical protocols for ccfDNA analysis.
  • To provide a guideline for implementing ccfDNA analysis in routine clinical practice.

Main Methods:

  • Literature review of studies on sample handling for ccfDNA analysis.
  • Analysis of published data and internal observations on preanalytical variables.
  • Evaluation of preanalytical steps from blood collection to DNA storage.

Main Results:

  • Preanalytical factors significantly influence ccfDNA concentration and fragmentation.
  • Specific preanalytical steps demonstrably affect ccfDNA analysis outcomes.
  • Optimal protocols were identified based on data analysis.

Conclusions:

  • Standardized preanalytical protocols are essential for reliable ccfDNA analysis.
  • The identified optimal protocols can guide the clinical implementation of ccfDNA testing.
  • Establishing guidelines will facilitate the translation of ccfDNA analysis into routine practice.