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Updated: May 10, 2026

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
Published on: August 20, 2021
The eukaryotic genome, its reads, and the unfinished assembly
José Fernando Muñoz1, Juan Esteban Gallo, Elizabeth Misas
1Cellular & Molecular Biology Unit, Corporación para Investigaciones Biológicas, Medellín, Colombia.
Abstract:
In recent years, readily affordable short read sequences provided by next-generation sequencing (NGS) have become longer and more accurate. This has led to a jump in interest in the utility of NGS-only approaches for exploring eukaryotic genomes. The concept of a static, 'finished' genome assembly, which still appears to be a faraway goal for many eukaryotes, is yielding to new paradigms. We here motivate an object-view concept where the raw reads are the main, fixed object, and assemblies with their annotations take a role of dynamically changing and modifiable views of that object.
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