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Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
Published on: March 13, 2018
Genomic mosaicism in two strains of Dengue virus type 3
Christian Julián Villabona-Arenas1, Anderson Fernandes de Brito, Paolo Marinho de Andrade Zanotto
1Laboratory of Molecular Evolution and Bioinformatics, Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Lineu Prestes 1734, CEP: 05508-000, São Paulo, SP, Brazil.
Abstract:
Recombination is a significant factor driving genomic evolution, but it is not well understood in Dengue virus. We used phylogenetic methods to search for recombination in 636 Dengue virus type 3 (DENV-3) genomes and unveiled complex recombination patterns in two strains, which appear to be the outcome of recombination between genotype II and genotype I parental DENV-3 lineages. Our findings of genomic mosaic structures suggest that strand switching during RNA synthesis may be involved in the generation of genetic diversity in dengue viruses.
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