Development of a novel FRET probe for the real-time determination of ceramidase activity
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Updated: May 10, 2026
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Quantitative FRET (Förster Resonance Energy Transfer) Analysis for SENP1 Protease Kinetics Determination
Published on: February 21, 2013
Krishna P Bhabak1, Anett Hauser, Susanne Redmer
1Department of Organic & Bioorganic Chemistry, Humboldt Universität zu Berlin, Institute for Chemistry, Brook-Taylor Str-2, 12489 Berlin, Germany.
Chembiochem : a European Journal of Chemical Biology
|June 4, 2013
Abstract:
Fretful novelty: We developed two novel doubly labelled fluorescent ceramide analogues that exhibit significant FRET and undergo hydrolysis by ceramidases. We present a fluorescent sphingolipid FRET probe that allows homogeneous ratiometric determination of enzyme activity in real-time.
