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Updated: May 10, 2026

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Published on: November 9, 2019
Carboligation reactions mediated by benzoylformate decarboxylase immobilized on a magnetic solid support
Bilsen Tural1, Servet Tural, Ayhan S Demir
1Department of Chemistry, Dicle University, Diyarbakir, Turkey. bilsentural@gmail.com
Abstract:
In this study, magnetic nanoparticles (Fe3 O4 , magnetite) with immobilized metal affinity ligands (MSS) were prepared and characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), fourier transform infrared spectroscopy (FTIR), and vibrating-sample magnetometer (VSM) methods for purification and immobilization of the histidine-tagged recombinant benzoylformate decarboxylase (BFD). The MSS support was shown to be eligible for selective binding of HIS-tagged BFD by SDS-page analysis. Loading capacity of the MSS support was determined as 43.6 ± 1.1 mg/g. The regeneration ability for protein binding was also studied. An immobilized BFD was tested to catalyze benzoin condensation and representative cross acyloin reaction. Conversion and enantiomeric excess values were comparable with that of free enzyme catalyzed reactions.
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