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Low concentration galactose determination in plasma adapted to the Cobas-Bio
M R Pudek1, A Jamani, V Bernstein
1Department of Pathology, Vancouver General Hospital, University of British Columbia, Canada.
Clinical Biochemistry
|June 1, 1990
Summary
This study presents an automated assay for measuring low plasma galactose concentrations, crucial for assessing liver blood flow. The new method is more precise and linear than manual techniques, offering faster results.
Area of Science:
- Biochemistry
- Clinical Chemistry
- Medical Diagnostics
Background:
- Functional liver blood flow is often assessed using galactose elimination. Measuring low plasma galactose concentrations is essential for this assessment.
- Existing manual methods for galactose measurement can be time-consuming and less precise.
Purpose of the Study:
- To adapt and validate an automated assay for measuring low plasma galactose concentrations using galactose dehydrogenase.
- To compare the performance of the automated assay with a manual galactose oxidase method.
Main Methods:
- An automated assay employing galactose dehydrogenase (EC 1.1.1.48) was adapted for the Cobas-Bio analyzer.
- Blood samples were collected in sodium fluoride/potassium oxalate tubes, eliminating the need for plasma deproteinization.
- Assay precision and linearity were evaluated using spiked plasma samples.
Main Results:
- The automated assay demonstrated high precision with between-run coefficients of variation (CVs) of 3.6% and 3.2% for plasma samples spiked with galactose.
- The automated assay exhibited a wider linearity range (0.04-1.10 mmol/L) compared to the manual method (0.06-0.56 mmol/L).
- The total assay time was reduced to 20 minutes.
Conclusions:
- The automated galactose dehydrogenase assay provides a precise, linear, and efficient method for measuring low plasma galactose concentrations.
- This assay is suitable for assessing functional liver blood flow and offers advantages over existing manual methods.