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Determination of Vaccine Immunogenicity Using Bovine Monocyte-Derived Dendritic Cells
Published on: May 19, 2023
Noncanonical dendritic cell differentiation and survival driven by a bacteremic pathogen
Brodie Miles1, Elizabeth Scisci, Julio Carrion
11.Georgia Regent University Augusta, 1120 15th St., GC-1335, Augusta, GA 30912, USA. chcutler@gru.edu.
Journal of Leukocyte Biology
|June 5, 2013
Summary
Porphyromonas gingivalis infection drives myeloid dendritic cell (mDC) expansion and differentiation via a novel pathway. This process, termed PDDC development, impacts immune homeostasis and inflammation.
Area of Science:
- Immunology
- Microbiology
- Cell Biology
Background:
- Maintaining blood dendritic cell (DC) homeostasis is crucial for preventing autoimmunity and managing infections.
- The mechanisms by which bacteremic pathogens influence DC homeostasis remain largely undefined.
Purpose of the Study:
- To investigate how the bacteremic pathogen Porphyromonas gingivalis regulates blood DC homeostasis.
- To identify the molecular pathways involved in P. gingivalis-induced DC expansion and differentiation.
Main Methods:
- Infection of human monocytes with P. gingivalis strains.
- Assessment of DC differentiation using flow cytometry and gene silencing (siRNA).
- Analysis of PDDC phenotype, phagocytic activity, and T cell stimulatory capacity.
- Evaluation of apoptosis and pyroptosis resistance in infected DCs.
Main Results:
- P. gingivalis infection promotes the differentiation of monocytes into myeloid DCs (MoDCs) through a DC-SIGN/mfa-1 dependent pathway, independent of canonical factors.
- These P. gingivalis-derived DCs (PDDCs) exhibit bona fide DC characteristics and can stimulate T cell proliferation.
- P. gingivalis-infected MoDCs display resistance to apoptosis and pyroptosis.
Conclusions:
- A novel, noncanonical pathway for DC differentiation (PDDC development) induced by P. gingivalis has been identified.
- This mechanism contributes to altered immune homeostasis and may promote chronic inflammation during asymptomatic bacteremia.
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