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Identification of an ascorbic acid metabolite among "uremic middle molecules"
P M Gallice1, J P Monti, D L Braguer
1Laboratoire de Biophysique, Faculté de Pharmacie, Marseille, France.
Clinical Chemistry
|July 1, 1990
Summary
Ascorbic acid 2-sulfate, a major uremic toxin component, does not inhibit microtubule formation. The inhibitory factor in the "2-5-3 fraction" is likely present in very small quantities.
Area of Science:
- Biochemistry
- Nephrology
- Cell Biology
Background:
- Uremic toxins are implicated in middle molecular mass range pathologies.
- The
- The
- 2-5-3 fraction
- was previously shown to inhibit microtubule formation.
Purpose of the Study:
- To identify the inhibitory component of the
- 2-5-3 fraction
- and test its effect on tubulin polymerization.
Main Methods:
- Nuclear magnetic resonance (NMR) spectroscopy
- Ultraviolet (UV) spectrometry
- Chromatographic analyses
- Testing of ascorbic acid 2-sulfate and its enantiomers on tubulin polymerization.
Main Results:
- Ascorbic acid 2-sulfate was identified as the main component of the
- 2-5-3 fraction
- using spectroscopic and chromatographic methods.
- Ascorbic acid 2-sulfate and its enantiomers did not inhibit tubulin polymerization.
Conclusions:
- Ascorbic acid 2-sulfate is not the primary factor responsible for the previously observed inhibition of microtubule formation.
- The
- 2-5-3 fraction
- likely contains a different inhibitory compound in trace amounts.