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Prevalence and molecular genotyping of group a rotaviruses in Iranian children

Mohammad Kargar1, Amin Reza Akbarizadeh

  • 1Department of Microbiology, Jahrom Branch, Islamic Azad University, Jahrom, Iran.

Indian Journal of Virology : an Official Organ of Indian Virological Society
|June 5, 2013
PubMed

Insights

Rotavirus group A is common in Iranian children hospitalized with diarrhea. Genotyping revealed G4 and non-typable strains were most frequent, providing data for vaccine development in infants.

Area of Science:

  • Pediatrics
  • Infectious Diseases
  • Vaccinology

Background:

  • Rotavirus is a primary cause of severe gastroenteritis in young children globally.
  • Despite available vaccines, rotavirus remains a significant public health concern, particularly during winter.
  • Limited vaccine uptake necessitates ongoing surveillance of rotavirus prevalence and genotypes.

Purpose of the Study:

  • To determine the prevalence of rotavirus infection in hospitalized children under five years old.
  • To identify the circulating rotavirus genotypes in Jahrom City, Iran.
  • To provide data for the development of effective rotavirus vaccines.

Main Methods:

  • Collected 163 stool samples from hospitalized children (<5 years) with severe diarrhea between 2009-2010.
  • Utilized enzyme immunoassay (EIA) to detect rotavirus group A antigen.
  • Employed nested reverse transcription-polymerase chain reaction (RT-PCR) for rotavirus genotype determination.

Main Results:

  • Rotavirus group A antigen was detected in 75 out of 163 (46%) samples.
  • The most prevalent genotypes were G4 (30.66%) and non-typable strains (30.66%), followed by G1 (17.33%) and G2 (13.34%).
  • The G1/G8 mixed infection was reported for the first time in Iran.

Conclusions:

  • Rotavirus group A infection is highly prevalent in hospitalized children with diarrhea in Jahrom, Iran.
  • The identified circulating genotypes, including G4 and non-typable strains, are crucial for regional vaccine strategies.
  • This study highlights the importance of continuous rotavirus surveillance for public health interventions in infants.

Keywords:
Molecular genotypingPrevalenceRT-PCRRotavirus

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