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Simple and Robust in vivo and in vitro Approach for Studying Virus Assembly
Published on: March 1, 2012
In Vitro Expression and Production of Antibody Against Cymbidium mosaic virus Coat Protein
A R Sherpa1, V Hallan, A A Zaidi
1Plant Virus Laboratory, Floriculture Division, Institute of Himalayan Bioresource Technology (CSIR), Post Box No. 6, Palampur, 176061 Himachal Pradesh India ; Department of Botany, West Bengal State University, Berunanpukuria, Malikapur, 24 Parganas (N), Kolkota, 700126 India.
Abstract:
Polyclonal rabbit antisera were produced using coat protein of Cymbidium mosaic virus (CymMV) Indian isolate expressed in E. coli as GST fusion. The expressed protein was purified by GST-fusion protein purification kit for use as an immunogen in rabbits. Antisera prepared in this manner reacted in double antibody sandwich enzyme-linked immunosorbent assay (DAS ELISA) with extract from CymMV-infected tissue. The results indicate that polyclonal antisera prepared from expressed CymMV coat proteins were useful for the detection of CymMV in an array of assays. The detection system developed is highly effective for detection of Indian strain of the virus in comparison to kits available in the international market.
