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Related Concept Videos

Electrospray Ionization (ESI) Mass Spectrometry01:12

Electrospray Ionization (ESI) Mass Spectrometry

Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
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In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
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Fabrication of a Dipole-assisted Solid Phase Extraction Microchip for Trace Metal Analysis in Water Samples
09:42

Fabrication of a Dipole-assisted Solid Phase Extraction Microchip for Trace Metal Analysis in Water Samples

Published on: August 7, 2016

Microchip emitter for solid-phase extraction-gradient elution-mass spectrometry.

Natalia Gasilova1, Liang Qiao, Dmitry Momotenko

  • 1Laboratoire d'Electrochimie Physique et Analytique, Ecole Polytechnique Fédérale de Lausanne, Station 6, CH-1015 Lausanne, Switzerland.

Analytical Chemistry
|June 5, 2013
PubMed
Summary
This summary is machine-generated.

A novel microchip electrospray emitter with a magnetic bead trap enables sensitive peptide detection. This solid-phase extraction-gradient elution-mass spectrometry (SPE-GEMS) method effectively enriches and analyzes low-abundance peptides.

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Mass Spectrometry

Background:

  • Direct mass spectrometry analysis often fails to detect low-abundance peptides.
  • Solid-phase extraction (SPE) is crucial for sample enrichment and purification.
  • Integrating SPE with mass spectrometry (MS) can improve detection limits.

Purpose of the Study:

  • To develop and demonstrate a microchip electrospray emitter with a magnetic bead trap for SPE-GEMS.
  • To enable sensitive preconcentration and sequential elution of peptides for MS analysis.
  • To detect low-abundance peptides in complex biological samples.

Main Methods:

  • Design of a microchip electrospray emitter with an integrated magnetic bead trap.
  • Utilized reverse-phase coated magnetic beads for peptide retention and enrichment.
  • Implemented stepwise gradient elution for sequential peptide release.
  • Coupled SPE with electrospray ionization mass spectrometry (ESI-MS).

Main Results:

  • Demonstrated efficient preconcentration of model peptides from 1 μM down to 10 nM.
  • Successfully performed sequential elution and MS analysis of peptides.
  • Analyzed fortified human blood serum and protein digests, detecting previously unobserved low-abundance peptides.
  • Validated the SPE-GEMS approach for complex biological matrices.

Conclusions:

  • The SPE-GEMS method offers effective sample desalting, enrichment, and sequential elution.
  • This integrated approach enhances the detection of low-abundance peptides without additional separation steps.
  • The developed microchip system provides a powerful tool for sensitive peptide analysis in complex samples.