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Updated: May 10, 2026
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Intravital Microscopy of Leukocyte-endothelial and Platelet-leukocyte Interactions in Mesenterial Veins in Mice
Published on: August 13, 2015
Measuring leukocyte-endothelial interactions in mice
Cold Spring Harbor Protocols
|June 5, 2013
Summary
Intravital imaging visualizes tumor pathophysiology by tracking leukocyte migration and interactions with blood vessels. This method quanties changes in leukocyte-endothelial interactions for cancer research.
Area of Science:
- Oncology
- Vascular Biology
- Biomedical Imaging
Background:
- Intravital imaging is crucial for understanding tumor pathophysiology.
- Vascular parameters like angiogenesis and leukocyte-endothelial interactions are key in tumors.
Purpose of the Study:
- To detail a protocol for intravital microscopy in mice.
- To quantify leukocyte-endothelial interactions within tumor vasculature.
Main Methods:
- Utilized single-photon and multiphoton laser-scanning microscopy.
- Administered Rhodamine-6G tracer via tail vein injection to label leukocytes.
- Monitored leukocyte migration and interactions with the endothelium.
Main Results:
- Successfully tracked Rhodamine-6G labeled leukocytes in mouse vasculature.
- Quantified rolling and adhering leukocytes, and total leukocyte flux.
- Demonstrated the ability to monitor leukocyte-endothelial interactions dynamically.
Conclusions:
- This protocol provides a robust method for studying tumor vascular dynamics.
- Intravital imaging of leukocyte-endothelial interactions offers insights into tumor pathophysiology.
- The technique is valuable for preclinical cancer research and therapeutic development.
