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Updated: May 10, 2026

Correlative Light and Electron Microscopy (CLEM) as a Tool to Visualize Microinjected Molecules and their Eukaryotic Sub-cellular Targets
Published on: May 4, 2012
Correlative light and electron microscopy for the analysis of cell division
Stefanie Redemann1, Thomas Müller-Reichert
1Structural Cell Biology Group, Experimental Center, Medical Faculty Carl Gustav Carus, University of Technology Dresden, Dresden 01307, Germany.
Correlative light and electron microscopy (CLEM) combines dynamic and ultrastructural imaging for cell biology. This approach offers enhanced insights into cellular processes like mitosis and cytokinesis compared to individual methods.
Area of Science:
- Cell Biology
- Microscopy Techniques
Background:
- Correlative light and electron microscopy (CLEM) is increasingly utilized in cell biology.
- CLEM integrates dynamic and ultrastructural information for comprehensive analysis.
Purpose of the Study:
- To summarize two correlative light and electron microscopy (CLEM) approaches.
- To highlight the application of CLEM in studying cell division.
Main Methods:
- Utilized correlative light and electron microscopy (CLEM).
- Focused on two specific CLEM methodologies.
Main Results:
- Demonstrated the combined power of light and electron microscopy.
- Provided insights into subcellular processes during mitosis and cytokinesis.
Conclusions:
- CLEM offers complementary information beyond individual imaging techniques.
- CLEM is a valuable tool for analyzing dynamic and ultrastructural cellular events.
Related Concept Videos
Two-Dimensional Microscopy in Microbiology
Three-Dimensional Microscopy in Microbiology
Overview of Electron Microscopy
Overview of Microscopy Techniques

