An in vitro model for spontaneous angiogenesis using rat mesenteric endothelial cells: possible therapeutic...

Abnormal obesity and the related diseases, such as diabetes and cardiovascular disease, are the main causes of mortality, around the world. A key feature of the adipogenesis and obesity is angiogenesis-dependent tissue growth accompanied with extracellular remodeling. In this way, suppression of angiogenesis may be a key point for preventing the adipogenesis.

In the present study, to provide a deeper insight to understand obesity and screening for more effective therapeutics, we have developed a three-dimensional in vitro model of microvessel formation under collagen matrix culture using endothelial cells, extracted from a suitable tissue.

In a successful approach for developing an angiogenesis model, the rat mesenteric microvascular endothelial cells (RMMECs) were isolated, coated on dextran beads and then suspended in collagen gel. Additionally, the proliferation as well as migration of endothelial cells were analyzed and compared with human umbilical vein endothelial cells (HUVECs).

RMMECs showed remarkable migration ability and had higher growth during the logarithmic growth phase, when compared with HUVECs. Also, no significance differences in morphogenesis were observed between HUVECs and RMMECs.

The model may be useful in providing insights to develop potential intervention strategies in vivo against obesity-related disorders. Targeting endothelial cells is an interesting and exciting possibility that may be raised in further investigations.