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Updated: May 10, 2026
Microfluidics in Assessing Platelet Function
Published on: November 8, 2024
Structure elucidation and inhibitory effects on human platelet aggregation of chlorogenic acid from Wrightia
Ripal P Amin1, Naveen Kunaparaju, Sunil Kumar
1Department of Pharmaceutical Sciences, College of Pharmacy and Health Professions, St. John’s University, Jamaica, NY 11439, USA. ripalamin@gmail.com
Background:
Interest in natural compounds as sources of potentially new treatment options is growing rapidly. Preliminary screening of many different plant extracts showed that Wrightia tinctoria acts as a potent human platelet aggregation inhibitor. The aim of the present study was to isolate and characterize the active compound responsible for potent inhibition of human platelet aggregation in vitro.
Methods:
A 70% ethanolic extract derived from W. tinctoria seeds was fractionated with chloroform followed by ethyl acetate. The ethyl acetate fraction was further fractionated and purified through a series of three successive column chromatographic separations using silica gel, Sephadex LH-20, and C-18 columns. Liquid chromatography coupled to negative electrospray ionization tandem mass spectrometry (LC-MS/MS) and nuclear magnetic resonance (NMR) studies were performed in the structure determination of the active phenolic compound present in the ethyl acetate fraction of W. tinctoria seeds.
Results:
A phenolic compound has been isolated and identified as chlorogenic acid by LC-MS/MS and NMR studies. Chlorogenic acid showed concentration-dependent inhibitory effect on collagen-induced platelet aggregation in vitro with an IC50 of 0.2363 μg/μl.
Conclusion:
The present data suggest that chlorogenic acid can be developed as potential antiplatelet agent in the treatment of cardiovascular diseases in diabetes mellitus.
