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Related Experiment Video

Updated: May 10, 2026

Single Synapse Indicators of Glutamate Release and Uptake in Acute Brain Slices from Normal and Huntington Mice
08:27

Single Synapse Indicators of Glutamate Release and Uptake in Acute Brain Slices from Normal and Huntington Mice

Published on: March 11, 2020

[Imaging non-synaptic transmission via glutamate].

Yohei Okubo1

  • 1Department of Pharmacology, Graduate School of Medicine, The University of Tokyo, Japan.

Brain and Nerve = Shinkei Kenkyu No Shinpo
|June 6, 2013
PubMed
Summary

Glutamate spillover, or non-synaptic transmission, was visualized using new fluorescent indicators. This study reveals how glutamate escapes synapses to regulate brain functions and activates receptors in vivo.

Area of Science:

  • Neuroscience
  • Cellular Biology
  • Biochemistry

Context:

  • Glutamate is the primary excitatory neurotransmitter in the mammalian brain.
  • Traditionally, glutamate transmission is viewed as point-to-point within synapses.
  • Extrasynaptic glutamate release (spillover) and its functional roles are increasingly recognized.

Purpose:

  • To investigate the spatiotemporal dynamics of extrasynaptic glutamate concentrations.
  • To overcome technological limitations in measuring glutamate spillover.
  • To visualize and characterize glutamate spillover in real-time.

Summary:

  • Developed and utilized a hybrid-type fluorescent glutamate indicator for imaging.
  • Demonstrated that glutamate spillover is induced by clustered synaptic activity.

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Multi-photon Intracellular Sodium Imaging Combined with UV-mediated Focal Uncaging of Glutamate in CA1 Pyramidal Neurons
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Published on: October 8, 2014

Related Experiment Videos

Last Updated: May 10, 2026

Single Synapse Indicators of Glutamate Release and Uptake in Acute Brain Slices from Normal and Huntington Mice
08:27

Single Synapse Indicators of Glutamate Release and Uptake in Acute Brain Slices from Normal and Huntington Mice

Published on: March 11, 2020

Combined Optogenetic and Freeze-fracture Replica Immunolabeling to Examine Input-specific Arrangement of Glutamate Receptors in the Mouse Amygdala
09:49

Combined Optogenetic and Freeze-fracture Replica Immunolabeling to Examine Input-specific Arrangement of Glutamate Receptors in the Mouse Amygdala

Published on: April 15, 2016

Multi-photon Intracellular Sodium Imaging Combined with UV-mediated Focal Uncaging of Glutamate in CA1 Pyramidal Neurons
10:29

Multi-photon Intracellular Sodium Imaging Combined with UV-mediated Focal Uncaging of Glutamate in CA1 Pyramidal Neurons

Published on: October 8, 2014

  • Showed spillover activates high-affinity glutamate receptors and occurs in vivo.
  • Observed sensory input-induced glutamate spillover in the cerebral cortex.

    • Impact:

    • Provides direct visualization of glutamate spillover dynamics.
    • Clarifies the features of non-synaptic glutamate transmission.
    • Enables direct observation of synaptic activity in live animals.
    • Offers insights into neural and glial functions regulated by glutamate spillover.