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Updated: May 10, 2026
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Use of the Protease Fluorescent Detection Kit to Determine Protease Activity
Published on: August 4, 2009
Fluorescence amplified detection of proteases by the catalytic activation of a semisynthetic sensor
Po-Ming Shih1, Tao-Kai Liu, Kui-Thong Tan
1Department of Chemistry, National Tsing Hua University, 101 Sec. 2, Kuang Fu Road, Hsinchu 30013, Taiwan, ROC.
Chemical Communications (Cambridge, England)
|June 6, 2013
Abstract:
A general approach to mimic the sensing scheme of allosteric enzymes is developed. Through the covalent labeling of a sulfonamide inhibitor to the enzyme HCAII via SNAP-tag protein, the enzyme is rendered inactive. Catalytic activation is triggered only when a protease is present to cleave the recognized peptide sequence.
