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Updated: May 10, 2026

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects
Published on: February 8, 2014
Digital holography simulations and experiments to quantify the accuracy of 3D particle location and 2D sizing using a
Daniel R Guildenbecher1, Jian Gao, Phillip L Reu
1Sandia National Laboratories, Albuquerque, New Mexico 87185, USA. drguild@sandia.gov
Abstract:
The accuracy of digital in-line holography to detect particle position and size within a 3D domain is evaluated with particular focus placed on detection of nonspherical particles. Dimensionless models are proposed for simulation of holograms from single particles, and these models are used to evaluate the uncertainty of existing particle detection methods. From the lessons learned, a new hybrid method is proposed. This method features automatic determination of optimum thresholds, and simulations indicate improved accuracy compared to alternative methods. To validate this, experiments are performed using quasi-stationary, 3D particle fields with imposed translations. For the spherical particles considered in experiments, the proposed hybrid method resolves mean particle concentration and size to within 4% of the actual value, while the standard deviation of particle depth is less than two particle diameters. Initial experimental results for nonspherical particles reveal similar performance.

