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Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

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Related Experiment Video

Updated: May 10, 2026

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography
11:21

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Published on: January 15, 2013

Snapshot 3D optical coherence tomography system using image mapping spectrometry.

Thuc-Uyen Nguyen1, Mark C Pierce, Laura Higgins

  • 1Department of Bioengineering, Rice University, Houston, Texas 77030, USA.

Optics Express
|June 6, 2013
PubMed
Summary
This summary is machine-generated.

A new 3D Optical Coherence Tomography system uses Image Mapping Spectrometry for faster imaging. This technology reduces motion artifacts, improving throughput for 3D sample visualization.

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Published on: October 2, 2021

Area of Science:

  • Biomedical Optics
  • Optical Imaging
  • Spectroscopy

Background:

  • Motion artifacts and limited throughput hinder 3D imaging in Optical Coherence Tomography (OCT).
  • Developing faster OCT systems is crucial for in-vivo and ex-vivo applications.

Purpose of the Study:

  • To develop a snapshot 3D Optical Coherence Tomography (OCT) system.
  • To utilize Image Mapping Spectrometry (IMS) for enhanced imaging speed and depth information acquisition.

Main Methods:

  • A snapshot 3D OCT system was engineered integrating Image Mapping Spectrometry.
  • The system captures a (x,y,λ) datacube of (85×356×117) for 3D sample visualization.

Main Results:

  • The system provides depth information (Z) at different spatial positions (XY) within a single camera integration time.
  • Achieved a transverse resolution of 13.4 μm and an axial resolution of 16.0 μm over a 400 μm depth.
  • Demonstrated potential to reduce motion artifacts and enhance imaging throughput.

Conclusions:

  • The developed snapshot 3D OCT system offers a promising approach for rapid volumetric imaging.
  • Theoretical analysis provides a roadmap for future system enhancements in imaging depth and resolution.