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Updated: May 10, 2026

Isolation of Glomeruli and In Vivo Labeling of Glomerular Cell Surface Proteins
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Isolating glomeruli from mice: A practical approach for beginners.

Xiaodan Liu1, Qiuling Fan, Gang Yang

  • 1Department of Nephrology, The First Affiliated Hospital, China Medical University, Shenyang, Liaoning 110001, P.R. China.

Experimental and Therapeutic Medicine
|June 6, 2013
PubMed
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Researchers developed a cost-effective Dynabead perfusion method for isolating pure, intact glomeruli from mice. This practical technique aids in studying glomerular diseases and proteomics, especially for beginners.

Area of Science:

  • Nephrology
  • Proteomics
  • Molecular Biology

Background:

  • Glomerular diseases require pure glomerular samples for mechanistic studies.
  • Existing isolation methods can be costly and technically demanding.
  • Dynabead perfusion offers a potential alternative for high-purity glomeruli isolation.

Purpose of the Study:

  • To develop a practical and cost-effective Dynabead perfusion method for isolating mouse glomeruli.
  • To ensure high purity and structural integrity of isolated glomeruli.
  • To facilitate research into glomerular diseases and proteomics.

Main Methods:

  • A modified Dynabead perfusion technique was applied to C57BL/6 mouse kidneys.
  • Glomeruli were isolated via kidney perfusion and compared to heart perfusion.
Keywords:
Dynabeadsglomeruluskidney perfusionproteomics

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Last Updated: May 10, 2026

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Published on: January 18, 2019

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  • Purity, structural integrity, protein yield, and cost-effectiveness were assessed.

    • Main Results:

    • The kidney perfusion method achieved a 100% success rate with 96.7±1.2% purity.
    • Isolated glomeruli remained intact with significant protein yields (45.6–55.8 μg).
    • The cost of kidney perfusion was 1/40th of heart perfusion.

    Conclusions:

    • The modified Dynabead perfusion is a practical, high-yield, and cost-effective method for isolating pure mouse glomeruli.
    • This technique supports research in glomerular diseases and proteomics.
    • The method is particularly beneficial for novice researchers.