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Published on: June 10, 2021
Rapid synthesis of isoprenoid diphosphates and their isolation in one step using either thin layer or flash
1Department of Biochemistry and Molecular Biology, University of South Florida, College of Medicine, Box 7,12 901 N. 30th Street, Tampa, FL 33612-4799, USA.
Abstract:
A rapid procedure for the preparation of short-chain (C s-C.) isoprenoid diphosphates is described . It is based on the method of Cornforth and Popjak [Methods Enzymol., 15 (1969) 359-390] which utilizes bis-triethylammonium phosphate in trichioroacetonitriie as the phosphorylating reagent . The reaction takes place in 15 min, and product isolation, previously requiring several steps, is done in a single step using either preparative thin-layer chromatography or flash chromatography on silica . From a single TLC plate, up to 50 μ mol of pure farnesyl diphosphate (i .e., ca. 20 mg) can be isolated, while up to 1200 μ mol can be isolated using a standard flash chromatography column.
