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Detection of a few metallo-protein molecules using color centers in nanodiamonds

A Ermakova1, G Pramanik, J-M Cai

  • 1Institut für Quantenoptik and IQST, ‡Institut für Organische Chemie III and IQST, §Institut für Theoretische Physik and IQST, and ∥Materialwissenschaftliche Elektronenmikroskopie and IQST, Albert-Einstein Allee 11, Universität Ulm , 89069 Ulm, Germany.

Nano Letters
|June 7, 2013
PubMed

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Different methods, such as visual observance of metal-ion indicators, spectroscopic techniques, and potentiometric methods, can determine the endpoint of an EDTA titration.
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a result, EDTA...