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Related Experiment Video

Updated: May 10, 2026

Expression of Fluorescent Proteins in Branchiostoma lanceolatum by mRNA Injection into Unfertilized Oocytes
09:31

Expression of Fluorescent Proteins in Branchiostoma lanceolatum by mRNA Injection into Unfertilized Oocytes

Published on: January 12, 2015

Amphioxus makes the cut-Again.

Ildikó M L Somorjai1, Hector Escrivà, Jordi Garcia-Fernàndez

  • 1Centre for Organismal Studies; University of Heidelberg; Heidelberg, Germany ; CNRS; UMR7232; Universite Pierre et Marie Curie Paris 06; Observatoire Oceanologique; Banyuls-sur-Mer; Paris, France ; Department de Genètica; Facultat de Biologia; Universitat de Barcelona; Barcelona, Spain.

Communicative & Integrative Biology
|June 7, 2013
PubMed
Summary

Amphioxus, a model organism, demonstrates significant adult regenerative capacity, particularly in tail regeneration. Wnt signaling plays a crucial role in this process, offering insights into vertebrate evolution and aging.

Keywords:
Branchiostoma lanceolatumWntamphioxusrepeated regeneration

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Area of Science:

  • * Evolutionary developmental biology
  • * Regenerative medicine
  • * Comparative genomics

Background:

  • * Cephalochordates, like amphioxus, serve as crucial models for understanding vertebrate evolutionary origins.
  • * Amphioxus exhibits remarkable adult regenerative capabilities, making it a valuable system for studying tissue repair.
  • * Previous research has highlighted the potential of amphioxus in regeneration studies.

Purpose of the Study:

  • * To investigate the molecular mechanisms underlying tail regeneration in the European amphioxus (Branchiostoma lanceolatum).
  • * To assess the regenerative capacity of amphioxus after repeated tail amputations.
  • * To explore the role of Wnt signaling in amphioxus tail regeneration.

Main Methods:

  • * Analysis of gene expression patterns, specifically focusing on Wnt5.
  • * Detection of β-catenin protein in regenerating tail tissues.
  • * Repeated amputation experiments on adult amphioxus specimens.

Main Results:

  • * Wnt5 gene expression and β-catenin protein presence were detected in the early bud-stage blastema during tail regeneration.
  • * Branchiostoma lanceolatum demonstrated sustained and effective regeneration following multiple tail amputations.
  • * These findings support the involvement of Wnt signaling in the regenerative process.

Conclusions:

  • * Wnt signaling is implicated in the extensive tail regenerative capacity of adult amphioxus.
  • * Amphioxus serves as a robust model for studying regeneration, with implications for understanding vertebrate regeneration and aging-related decline.
  • * The study contributes to the fields of evolutionary biology and regenerative medicine.