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Updated: May 10, 2026
Double Whole Mount in situ Hybridization of Early Chick Embryos
Published on: October 27, 2008
Temporospatial expression of Dmrt1 in chicken urogenital system (Gallus gallus) using whole mount in situ
Y Yang1, Ping Gong, Yan P Feng
1Huazhong Agricultural University Key Lab of Agricultural Animal Genetics, Wuhan Hubei, People's Republic of China.
Abstract:
Doublesex and mab-3-related transcription factor 1 (Dmrt1) is a Z-linked gene that putatively determines the phenotype of gonads in birds. The sex differential expression of Dmrt1 was examined using wholemount in situ hybridization (WISH) in the urogenital systems during embryogenesis. The results revealed that Dmrt1 showed dimorphic expression in chicken gonads, which increased from day 6.5 to day 10.5. The expression of Dmrt1 in male (ZZ) gonads was not twice as much as in female (ZW) gonads, suggesting the existence of other regulatory mechanisms in addition to Z chromosome dosage effect.
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