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Histone Variants at the Centromere02:30

Histone Variants at the Centromere

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Evolution driven structural changes in CENP-E motor domain.

Ambuj Kumar1, Balu Kamaraj, Rao Sethumadhavan

  • 1Bioinformatics Division, School of Bio Sciences and Technology, Vellore Institute of Technology University, Vellore 632014, Tamil Nadu, India.

Interdisciplinary Sciences, Computational Life Sciences
|June 7, 2013
PubMed
Summary
This summary is machine-generated.

Genetic evolution drives biochemical changes, leading to new traits. This study reveals increased stability in the CENP-E motor domain from tarsiers to humans, highlighting evolving cell cycle proteins.

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Published on: December 28, 2021

Area of Science:

  • Evolutionary biology
  • Molecular biology
  • Biochemistry

Background:

  • Genetic evolution drives biochemical changes and the development of novel functional traits.
  • Phylogenetic analysis is crucial for understanding genetic relatedness and evolutionary pathways among species.
  • Centromere-associated protein-E (CENP-E) is essential for cell cycle regulation and kinetochore protein recruitment.

Purpose of the Study:

  • To investigate evolution-driven structural and functional alterations in the CENP-E motor domain across the primate lineage.
  • To analyze changes in ATP binding affinity and conformational dynamics of the human CENP-E motor domain compared to other primates.

Main Methods:

  • Employed molecular dynamics simulations and computational chemistry techniques.
  • Utilized metrics such as Root Mean Square Deviation (RMSD), Root Mean Square Fluctuation (RMSF), Radius of Gyration (Rg), and Principal Component Analysis (PCA).

Main Results:

  • Computational analyses indicated a progressive increase in stability of the CENP-E motor domain from tarsiers to humans.
  • Observed conformational deviations and changes in ATP binding affinity were quantified across primate species.

Conclusions:

  • The study demonstrates a gain in stability within the CENP-E motor domain during primate evolution.
  • Highlights the utility of theoretical approaches in simultaneously assessing structural, functional, and dynamic aspects of protein evolution, particularly for cell cycle proteins.