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Updated: May 10, 2026

DNA Methylation: Bisulphite Modification and Analysis

DNA Methylation: Bisulphite Modification and Analysis

Published on: October 21, 2011

DNA methylation analysis.

T von Känel¹, Andreas R Huber

¹Centre of Laboratory Medicine, Kantonsspital Aarau AG, Switzerland. thomas.vonkaenel@ksa.ch

Swiss Medical Weekly

|June 7, 2013

Summary

This summary is machine-generated.

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DNA methylation, a key epigenetic regulator, is crucial for diagnosing imprinting disorders and advancing cancer diagnostics. Methylation biomarker assays are becoming standard in lab medicine, though challenges remain for routine cancer applications.

Area of Science:

Epigenetics and Molecular Biology
Genomics and Bioinformatics
Laboratory Medicine and Diagnostics

Background:

DNA methylation, the epigenetic modification of cytosines and adenosines, plays a vital role in regulating gene expression.
Aberrant DNA methylation patterns are linked to human malignancies like cancer and imprinting disorders.
These methylation changes present promising targets for diagnostic and prognostic applications in medicine.

Purpose of the Study:

To provide an introduction to the biology of DNA methylation.
To present the application of DNA methylation biomarkers in laboratory medicine.
To review current DNA methylation analysis assays for diagnostic purposes.

Main Methods:

Review of existing literature on DNA methylation biology and assays.

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Last Updated: May 10, 2026

DNA Methylation: Bisulphite Modification and Analysis

DNA Methylation: Bisulphite Modification and Analysis

Published on: October 21, 2011

Targeted DNA Methylation Analysis by Next-generation Sequencing

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Published on: September 18, 2020

Analysis of assays based on bisulphite treatment.

Analysis of assays based on methylation-sensitive restriction enzyme digestion.

Main Results:

DNA methylation assays are established for imprinting disorder diagnosis.
Methylation biomarkers show potential to significantly advance cancer diagnostics and prognostics.
Challenges exist in the routine implementation of cancer-related DNA methylation assays.

Conclusions:

DNA methylation analysis is a powerful tool in molecular diagnostics.
Established assays are effective for imprinting disorders, with ongoing development for cancer.
Further refinement is needed for widespread clinical adoption of cancer methylation assays.