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Related Experiment Video

Updated: May 10, 2026

A Magnetic Resonance Imaging Protocol for Stroke Onset Time Estimation in Permanent Cerebral Ischemia
09:59

A Magnetic Resonance Imaging Protocol for Stroke Onset Time Estimation in Permanent Cerebral Ischemia

Published on: September 16, 2017

Vascular changes after stroke in the rat: a longitudinal study using optimized magnetic resonance imaging.

Philipp Boehm-Sturm1, Tracy D Farr, Joanna Adamczak

  • 1In-Vivo-NMR Laboratory, Max Planck Institute for Neurological Research, Cologne, Germany.

Contrast Media & Molecular Imaging
|June 7, 2013
PubMed
Summary

Stroke causes changes in brain blood vessels, but the timing is unclear. Optimized steady-state contrast-enhanced MRI (SSCE MRI) in rats revealed decreased microvessel density and increased vessel size after stroke, with no late-stage angiogenesis detected.

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Performing Permanent Distal Middle Cerebral with Common Carotid Artery Occlusion in Aged Rats to Study Cortical Ischemia with Sustained Disability
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Optimized System for Cerebral Perfusion Monitoring in the Rat Stroke Model of Intraluminal Middle Cerebral Artery Occlusion
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Optimized System for Cerebral Perfusion Monitoring in the Rat Stroke Model of Intraluminal Middle Cerebral Artery Occlusion

Published on: February 17, 2013

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Last Updated: May 10, 2026

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A Magnetic Resonance Imaging Protocol for Stroke Onset Time Estimation in Permanent Cerebral Ischemia

Published on: September 16, 2017

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Optimized System for Cerebral Perfusion Monitoring in the Rat Stroke Model of Intraluminal Middle Cerebral Artery Occlusion
12:15

Optimized System for Cerebral Perfusion Monitoring in the Rat Stroke Model of Intraluminal Middle Cerebral Artery Occlusion

Published on: February 17, 2013

Area of Science:

  • Neuroscience
  • Radiology
  • Vascular Biology

Background:

  • Stroke-induced reduction in blood flow causes cell death and triggers pro-angiogenic responses and vascular remodeling.
  • The precise temporal dynamics of vascular alterations following stroke remain incompletely understood.
  • Understanding these changes is crucial for developing effective stroke therapies.

Purpose of the Study:

  • To optimize steady-state contrast-enhanced magnetic resonance imaging (SSCE MRI) for tracking dynamic vascular changes post-stroke.
  • To investigate the spatio-temporal profile of vascular remodeling for up to 4 weeks after transient middle cerebral artery occlusion (MCAO) in rats.
  • To correlate MRI findings with histological evidence of endothelial cell proliferation and angiogenesis.

Main Methods:

  • Optimized SSCE MRI protocol to assess hemodynamic parameters including relative cerebral blood volume (rCBV), microvessel density, and relative vessel size.
  • Longitudinal MRI scans were performed on rats up to 28 days post-MCAO.
  • 5-Bromo-2'deoxyuridine (BrdU) labeling was used to identify proliferating cells, particularly endothelial cells, during the first week post-stroke.

Main Results:

  • SSCE MRI demonstrated a significant decrease in microvessel density and an increase in vessel size within the ischemic striatum after MCAO.
  • Histological analysis confirmed persistently reduced microvessel density at 28 days post-stroke.
  • Endothelial cell proliferation was observed during the first week post-MCAO, but late-stage angiogenesis (increased vessel density) was not detected.

Conclusions:

  • The optimized SSCE MRI protocol effectively captures dynamic changes in cerebral vasculature following stroke.
  • Stroke induces immediate vascular alterations including reduced microvessel density and enlarged vessels, with early endothelial proliferation.
  • Late-stage angiogenesis does not appear to significantly increase microvessel density in the weeks following MCAO in this model.