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Effect of Leishmania major on human polymorphonuclear leucocyte function in vitro
A S al Tuwaijri1, I A al Mofleh, A A Mahmoud
1Department of Physiology, College of Medicine, King Saud University, Riyadh, Saudi Arabia.
Journal of Medical Microbiology
|July 1, 1990
Summary
Leishmania major parasite antigens significantly inhibit human polymorphonuclear leucocyte (PMNL) respiratory burst function. This inhibition occurred without affecting PMNL viability, suggesting a specific immune modulation mechanism.
Area of Science:
- Immunology
- Parasitology
- Cellular Biology
Background:
- Leishmania major is a protozoan parasite causing leishmaniasis.
- Polymorphonuclear leucocytes (PMNLs) are crucial immune cells involved in fighting infections.
- Understanding parasite-host interactions is key to developing effective treatments.
Purpose of the Study:
- To investigate the impact of Leishmania major antigens on human PMNL function.
- To assess the effect on the respiratory burst activity of PMNLs.
- To determine if parasite antigen concentration or type influences PMNL response.
Main Methods:
- Incubation of isolated human PMNLs with various concentrations of L. major antigens.
- Assessment of PMNL respiratory burst using Luminol-dependent chemiluminescence.
- Evaluation of PMNL viability post-incubation with parasite antigens.
Main Results:
- All tested Leishmania antigens inhibited PMNL respiratory burst by 35-64%.
- PMNL viability remained unaffected by antigen concentration or type.
- Oxygen free radical scavengers amplified the inhibitory effect of the antigens on PMNLs.
Conclusions:
- Leishmania major antigens possess potent inhibitory effects on human PMNL respiratory burst.
- The observed inhibition does not stem from direct toxicity to PMNLs.
- Parasite antigens may modulate innate immune responses, potentially aiding parasite survival.