Analysis of finit in urine sample using thin layer chromatography.

Area of Science:

• Analytical Chemistry
• Environmental Science
• Toxicology

Background:

• Finit is a widely used household insecticide requiring routine analysis.
• Current analytical methods like Gas Liquid Chromatography (GLC) and High-Performance Liquid Chromatography (HPLC) can be complex and costly.
• There is a need for a simpler, more accessible method for Finit detection, particularly in biological samples like urine.

Purpose of the Study:

• To develop and validate a novel Thin Layer Chromatography (TLC) method for the quantitative analysis of Finit in human urine samples.
• To establish an inexpensive, accurate, and non-destructive analytical technique for Finit detection.
• To improve the sensitivity and reproducibility of Finit analysis in biological matrices.

Main Methods:

• Finit was extracted from urine samples using established solvent extraction techniques.
• Extracted Finit was subsequently analyzed using Thin Layer Chromatography (TLC).
• Detection sensitivity was enhanced using various reagents including palladium chloride, silver nitrate, iodine vapor, and bromophenol blue.

Main Results:

• The developed TLC method successfully identified and quantified Finit in urine samples.
• The use of palladium chloride, silver nitrate, iodine vapor, and bromophenol blue significantly increased detection sensitivity.
• The method proved to be simple, inexpensive, accurate, and non-destructive, yielding reproducible results.

Conclusions:

• Thin Layer Chromatography (TLC) provides a viable and advantageous alternative for the analysis of Finit in urine.
• The developed method offers enhanced sensitivity and accuracy while maintaining simplicity and cost-effectiveness.
• This technique allows for reliable and reproducible monitoring of Finit exposure in biological samples.