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Wide-area scanner for high-speed atomic force microscopy.

Hiroki Watanabe¹, Takayuki Uchihashi, Toshihide Kobashi

¹Department of Physics, College of Science and Engineering, Kanazawa University, Kanazawa 920-1192, Japan.

The Review of Scientific Instruments

|June 8, 2013

Summary

This summary is machine-generated.

High-speed atomic force microscopy (HS-AFM) now images larger samples. A new wide-area scanner enables visualization of dynamic processes in live cells with unprecedented scan ranges.

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Area of Science:

Biophysics
Microscopy
Nanotechnology

Background:

High-speed atomic force microscopy (HS-AFM) allows visualization of protein dynamics.
Current HS-AFM systems have limited scan ranges (~1x4 μm), restricting observation of larger biological samples like cells.

Purpose of the Study:

To develop a wide-area scanner for HS-AFM capable of imaging larger biological samples.
To overcome the limitations of existing HS-AFM scan ranges for observing cellular dynamics.

Main Methods:

A leverage mechanism was employed to magnify piezoelectric actuator displacements, creating a wide-area scanner with a ~46x46 μm scan range.
Feed-forward inverse compensation and rounded-vertex triangular scan signals were used to suppress vibrations from fast X-scanner movements.
Polynomial-approximation-based open-loop control was implemented to eliminate nonlinearity and hysteresis in piezoelectric actuator displacements and scanner interference.

Main Results:

The developed wide-area scanner achieved a maximum XY scan range of approximately 46x46 μm.
A scan speed of 6.3 mm/s in the X-direction was reached for scan sizes up to ~40 μm.
The system successfully demonstrated video imaging of dynamic processes in live bacterial and eukaryotic cells.

Conclusions:

The new wide-area scanner significantly expands the capabilities of HS-AFM for observing dynamic processes in live cells.
This advancement enables high-resolution, dynamic imaging of larger biological systems previously inaccessible to HS-AFM.
The developed technology opens new avenues for studying cellular mechanics and dynamics at the nanoscale.