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Updated: May 10, 2026
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Generation of Monocyte-Derived Dendritic Cells with Differing Sialylated Phenotypes
Published on: October 20, 2023
Glycoform remodeling generates a synthetic T cell phenotype
Chunxia Zou1, Ravi S Loka, Yi Zhang
1Alberta Glycomics Centre, Department of Chemistry, University of Alberta, Edmonton, Alberta T6G 2G2, Canada.
Bioconjugate Chemistry
|June 8, 2013
Summary
Altering cell surface glycans, specifically galactose epitopes on CD45, reprograms T cell responses to stimuli. This chemical strategy modifies cellular phenotypes by changing lectin binding sites.
Area of Science:
- Glycobiology
- Cellular immunology
- Chemical biology
Background:
- Cellular responses to stimuli are influenced by protein glycosylation.
- Modifying cell surface glycans can alter cellular phenotypes and functions.
Purpose of the Study:
- To develop a chemical strategy for modifying T cell glycoforms.
- To investigate the impact of glycoform modification on cellular responses to stimulation.
- To identify specific protein targets involved in altered cellular responses.
Main Methods:
- Chemical modification of cellular glycans using metabolic labeling.
- Targeting the glycoprotein receptor CD45 for glycan alteration.
- Assessing cellular responses to lectin stimulation and receptor cross-linking.
Main Results:
- Chemical modification of T cell glycans resulted in altered cellular responses.
- Modification of CD45 phosphatase's glycoform was identified as key to observed response differences.
- Increased galactose epitopes on lymphoma cell line glycocalyx enhanced response to lectin stimulation via CD45.
Conclusions:
- Metabolic labeling enables reprogramming of cellular responses through glycan modification.
- Altering the number of lectin binding sites on the cell surface impacts cellular signaling.
- Targeting CD45 glycosylation offers a novel approach to modulate immune cell function.
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