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Related Concept Videos

RNA Editing02:23

RNA Editing

RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
Since the...

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Related Experiment Video

Updated: May 10, 2026

A Nonsequencing Approach for the Rapid Detection of RNA Editing
08:50

A Nonsequencing Approach for the Rapid Detection of RNA Editing

Published on: April 21, 2022

REDItools: high-throughput RNA editing detection made easy.

Ernesto Picardi1, Graziano Pesole

  • 1Department of Biosciences, Biotechnology and Biopharmaceutics, University of Bari, 70125 Bari, Italy. ernesto.picardi@uniba.it

Bioinformatics (Oxford, England)
|June 8, 2013
PubMed
Summary
This summary is machine-generated.

Detecting RNA editing sites from massive sequencing data is difficult. REDItools, a new suite of Python scripts, offers a high-throughput computational tool for investigating RNA editing using next-generation sequencing data.

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Related Experiment Videos

Last Updated: May 10, 2026

A Nonsequencing Approach for the Rapid Detection of RNA Editing
08:50

A Nonsequencing Approach for the Rapid Detection of RNA Editing

Published on: April 21, 2022

RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes
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Published on: July 22, 2014

A Rapid High-throughput Method for Mapping Ribonucleoproteins (RNPs) on Human pre-mRNA
13:00

A Rapid High-throughput Method for Mapping Ribonucleoproteins (RNPs) on Human pre-mRNA

Published on: December 2, 2009

Area of Science:

  • Bioinformatics
  • Computational Biology
  • Genomics

Background:

  • RNA editing site detection from massive sequencing data is a significant challenge.
  • Existing methodologies lack dedicated computational tools for high-throughput analysis.

Purpose of the Study:

  • To introduce REDItools, a novel suite of Python scripts.
  • To provide a computational solution for high-throughput investigation of RNA editing.

Main Methods:

  • Development of a suite of Python scripts named REDItools.
  • Utilizing next-generation sequencing data for RNA editing analysis.

Main Results:

  • REDItools enables high-throughput investigation of RNA editing.
  • Addresses the challenge of reliable RNA editing site detection.

Conclusions:

  • REDItools is a freely available computational tool for RNA editing research.
  • Facilitates the analysis of massive sequencing data for RNA editing site identification.