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Related Concept Videos

Mechanically-gated Ion Channels01:12

Mechanically-gated Ion Channels

Mechanically-gated ion channels are proteins found in eukaryotic and prokaryotic cell membranes that open in response to mechanical stress. Tension, compression, swelling, and shear stress can alter the conformation of the protein, opening a transmembrane channel that allows the passage of ions for signal transmission. In eukaryotes, mechanically-gated channels are distributed in several regions like the neurons, lungs, skin, bladder, and heart, where they play critical roles in numerous...
Mechanically-gated Ion Channels01:12

Mechanically-gated Ion Channels

Mechanically-gated ion channels are proteins found in eukaryotic and prokaryotic cell membranes that open in response to mechanical stress. Tension, compression, swelling, and shear stress can alter the conformation of the protein, opening a transmembrane channel that allows the passage of ions for signal transmission. In eukaryotes, mechanically-gated channels are distributed in several regions like the neurons, lungs, skin, bladder, and heart, where they play critical roles in numerous...
Channel Rhodopsins01:11

Channel Rhodopsins

Most organisms use photoreceptors to sense and respond to light. Examples of photoreceptors include bacteriorhodopsins and bacteriophytochromes in some bacteria, phytochromes in plants, and rhodopsins in the photoreceptor cells of the vertebral retina. The light-sensitive property of these receptors is because of the bound chromophores, such as bilin in the phytochromes and retinal in the rhodopsins.
Rhodopsins belong to the family of cell surface proteins called G-protein coupled receptors,...
Ion Channels01:19

Ion Channels

The movement of ions like sodium, potassium, and calcium into and out of the cell is essential to maintain the electrochemical gradient in living cells. The ion channels—a class of membrane transport proteins—help maintain this ionic gradient for the smooth functioning of physiological activities such as maintaining cell size and volume, conducting nerve impulses, and gas and nutrient exchange.
Ion channels are specialized integral membrane proteins on the plasma membrane that allow specific...
Fixation and Sectioning01:03

Fixation and Sectioning

Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
G-Protein Gated Ion Channels01:21

G-Protein Gated Ion Channels

GPCRs are primarily responsible for our sense of smell, taste, and vision.  The binding of a sensory stimulus activates GPCR to stimulate effector proteins, many of which are ion channels in the sensory organs. GPCRs modulate the opening and closing of the target ion channels either directly by binding them, or by releasing second messengers that activate these channels. As ions move across the membrane, the membrane potential is altered, which induces an appropriate response.
Sensory organs,...

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Related Experiment Video

Updated: May 10, 2026

Optical Monitoring of Living Nerve Terminal Labeling in Hair Follicle Lanceolate Endings of the Ex Vivo Mouse Ear Skin
11:29

Optical Monitoring of Living Nerve Terminal Labeling in Hair Follicle Lanceolate Endings of the Ex Vivo Mouse Ear Skin

Published on: April 5, 2016

Antimicrobial dyes and mechanosensitive channels.

Ramiz A Boulos1

  • 1School of Chemical and Physical Sciences, Flinders University, Bedford Park, SA, 5042, Australia. ramiz.boulos@flinders.edu.au

Antonie Van Leeuwenhoek
|June 8, 2013
PubMed
Summary

Antimicrobial dyes offer a forgotten solution to combat drug-resistant bacteria. Targeting the mechanosensitive ion channel of large conductance (MscL) presents a novel approach for developing new antimicrobial agents.

Area of Science:

  • Microbiology
  • Drug Discovery
  • Bacterial Physiology

Background:

  • The rise of antibiotic resistance necessitates novel antimicrobial strategies.
  • Antimicrobial dyes, historically overlooked, represent a potential resource against resistant bacteria.
  • The mechanosensitive ion channel of large conductance (MscL) is a conserved bacterial target.

Purpose of the Study:

  • To explore the potential of antimicrobial dyes as agents against drug-resistant bacteria.
  • To investigate the mechanosensitive ion channel of large conductance (MscL) as a target for antimicrobial dye action.
  • To combine dye mechanism exploration with the MscL target for novel therapeutic opportunities.

Main Methods:

  • Literature review on antimicrobial dye activity and mechanisms.

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Application of Membrane and Cell Wall Selective Fluorescent Dyes for Live-Cell Imaging of Filamentous Fungi
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Last Updated: May 10, 2026

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Multifunctional, Micropipette-based Method for Incorporation And Stimulation of Bacterial Mechanosensitive Ion Channels in Droplet Interface Bilayers
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  • Analysis of existing evidence on triphenylmethane dyes and MscL channel interaction.
  • Proposing a combined research approach for future studies.

    • Main Results:

    • Antimicrobial dyes are an underutilized class of compounds with potential against resistant bacteria.
    • Evidence suggests triphenylmethane dyes can target the MscL channel.
    • A dual approach of studying dye mechanisms and the MscL target is promising.

    Conclusions:

    • Antimicrobial dyes can serve as standalone agents or adjuvants to combat resistant bacterial infections.
    • The MscL channel is a viable and conserved target for antimicrobial dye development.
    • Further research combining dye mechanisms and MscL targeting holds significant therapeutic promise.