Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MAPK Signaling Cascades01:07

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors are of three kinds RI, RII, and RIII. The RI...
cAMP-dependent Protein Kinase Pathways01:25

cAMP-dependent Protein Kinase Pathways

Cyclic Adenosine Monophosphate (cAMP) is an essential second messenger that activates protein kinase A (PKA) and regulates various biological processes. A single epinephrine molecule binds to GPCR and activates several heterotrimeric G proteins, each stimulating multiple adenylyl cyclase, amplifying the signal, and synthesizing large numbers of cAMP molecules. Small changes in cAMP concentration affect PKA activity. The binding of four cAMP molecules induces a conformational change in PKA,...
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a rapamycin-insensitive companion...
The JAK-STAT Signaling Pathway01:20

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...
Amplifying Signals via Enzymatic Cascade01:22

Amplifying Signals via Enzymatic Cascade

When a ligand binds to a cell-surface receptor, the receptor's intracellular domain changes shape, which may either activate its enzyme function or allow its binding to other molecules. The initial signal is amplified by most signal transduction pathways. This means that a single ligand molecule can activate multiple molecules of a downstream target. Proteins that relay a signal are most commonly phosphorylated at one or more sites, activating or inactivating the protein. Kinases catalyze the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Whole-genome resequencing analysis reveals involvement of OfCOL4 (CONSTANS-like 4) in regulation of leaf bleaching in Osmanthus fragrans.

Gene·2026
Same author

Abrupt permafrost thaw drives exceptional carbon release across the Tibetan Plateau.

Nature communications·2026
Same author

The Rise in Carbapenem-Resistant <i>Acinetobacter baumannii</i> and the Emergence of Eravacycline as a Treatment Strategy: A Narrative Review.

Pathogens (Basel, Switzerland)·2026
Same author

Leaf-Inspired Layered Hydrogel with Spatially Selective Ag/Ag<sub>2</sub>S Formation for Photothermal Antibacterial Therapy and Strain Sensing.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

Targeting heparanase-mediated glycocalyx degradation as a novel strategy to inhibit EMT-driven pulmonary fibrosis.

International immunopharmacology·2026
Same author

TeCYC2c functions as a hub protein in the CYC2 gene cluster to regulate ray floret development in marigold (Tagetes erecta).

The Plant journal : for cell and molecular biology·2026

Related Experiment Video

Updated: May 10, 2026

Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis
07:26

Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis

Published on: April 1, 2022

Chrysin promotes osteogenic differentiation via ERK/MAPK activation.

Wenfeng Zeng1, Yan Yan, Fayun Zhang

  • 1Protein & Peptide Pharmaceutical Laboratory, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.

Protein & Cell
|June 8, 2013
PubMed
Summary

The flavonoid chrysin promotes osteogenic differentiation in bone cells by activating ERK1/2 signaling. This suggests chrysin

More Related Videos

Induction and Analysis of Epithelial to Mesenchymal Transition
10:37

Induction and Analysis of Epithelial to Mesenchymal Transition

Published on: August 27, 2013

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development
09:32

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development

Published on: June 15, 2017

Related Experiment Videos

Last Updated: May 10, 2026

Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis
07:26

Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis

Published on: April 1, 2022

Induction and Analysis of Epithelial to Mesenchymal Transition
10:37

Induction and Analysis of Epithelial to Mesenchymal Transition

Published on: August 27, 2013

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development
09:32

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development

Published on: June 15, 2017

Area of Science:

  • Biochemistry
  • Cell Biology
  • Pharmacology

Background:

  • Osteogenesis is crucial for bone health and osteoporosis treatment.
  • Flavonoids, like chrysin, possess anti-inflammatory properties with potential therapeutic applications.
  • Understanding the molecular mechanisms of osteogenesis is key to developing new bone-building therapies.

Purpose of the Study:

  • To investigate the effect of the flavonoid chrysin on osteogenic differentiation in preosteoblast cells.
  • To elucidate the signaling pathways involved in chrysin-induced osteogenesis.

Main Methods:

  • Utilized MC3T3-E1 preosteoblast cell line.
  • Assessed osteogenic differentiation markers (Runx2, Osx, Col1A1, OCN, OPN) and mineralized nodule formation.
  • Investigated the role of mitogen-activated protein kinases (MAPKs) including ERK1/2, JNK, and p38.
  • Employed specific inhibitors (U0126, PD98059, ICI182780) to block signaling pathways.

Main Results:

  • Chrysin alone induced osteogenic differentiation in MC3T3-E1 cells.
  • Promoted expression of key osteogenic transcription factors and bone markers.
  • Enhanced mineralization of extracellular matrix.
  • Chrysin preferentially activated the ERK1/2 pathway, not JNK or p38.
  • Inhibiting ERK1/2 or estrogen receptor (ER) blocked chrysin's osteogenic effects.

Conclusions:

  • Chrysin effectively promotes osteogenesis in a manner dependent on ERK1/2 activation and involvement of the estrogen receptor.
  • Chrysin demonstrates significant potential as a therapeutic agent for osteoporosis prevention and treatment.