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Tm(DOTP)5-: a 23Na+ shift agent for perfused rat hearts
D C Buster1, M M Castro, C F Geraldes
1Department of Chemistry, University of Texas, Dallas, Richardson 75083-0688.
Magnetic Resonance in Medicine
|July 1, 1990
Summary
A new thulium(III) complex, Tm(DOTP)5-, effectively distinguishes sodium-23 (23Na+) NMR signals inside and outside heart cells. This novel agent offers an alternative to existing dysprosium(III) complexes for cardiac research.
Area of Science:
- Biomedical Engineering
- Nuclear Magnetic Resonance (NMR) Spectroscopy
- Cardiovascular Physiology
Background:
- Distinguishing intracellular and extracellular sodium-23 (23Na+) is crucial for understanding cardiac function.
- Current methods using agents like Dy(TTHA)3- have limitations.
- Development of novel shift agents is needed for improved 23Na+ NMR studies.
Purpose of the Study:
- To introduce and evaluate Tm(DOTP)5- as a novel 23Na+ NMR shift agent.
- To compare the efficacy of Tm(DOTP)5- with the established Dy(TTHA)3-.
- To assess the utility of Tm(DOTP)5- in perfused rat hearts.
Main Methods:
- Synthesis and characterization of the thulium(III) complex Tm(DOTP)5-.
- Application of Tm(DOTP)5- as a shift agent in 23Na+ NMR spectroscopy.
- Direct comparison with Dy(TTHA)3- using perfused rat heart models.
Main Results:
- Tm(DOTP)5- successfully induced shifts in 23Na+ NMR resonances.
- The agent enabled discrimination between intra- and extracellular 23Na+ signals.
- Performance of Tm(DOTP)5- was evaluated against Dy(TTHA)3-.
Conclusions:
- Tm(DOTP)5- is a viable and effective 23Na+ shift agent for cardiac NMR.
- This novel agent provides a valuable tool for studying sodium ion dynamics in the heart.
- Tm(DOTP)5- represents a promising alternative for cardiovascular research applications.