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[Analysis of antimicrobial effect of MTAD solution in infected root canal system using PCR technique]
Introduction:
Clinically acceptable antiseptic should possess organolithic-mineralolithic properties and antimicrobial efficacy, and should be non-toxic.
Objective:
The aim of the paper was to assess the presence of genomes of the most common microorganisms (Porphyromonas gingivalis, Agregatibacter actinomycetemcomitans, Tanerella forsythensis, Prevotella intermedia, Treponema denticola and Enterococcus faecalis) in infected tooth root canals before and after rinsing with solution of doxycycline, citric acid and detergent Tween-80 (MTAD) in patients with clinically diagnosed primary apex periodontitis.
Methods:
The content of primarily infected canals before and after using the MTAD solution was used as a biological material in which the presence of microorganisms DNA was proved. For the detection of bacterial genome the multiplex PCR technique was applied.
Results:
The percentage of positive samples before canal treatment was 100%. In infected root canals E. faecalis was most dominant (37%). In a relatively high percentage we detected P. intermedia (25%), A. actinomycetemcomitans (20%), T. denticola (17%), T. forsythensis (15%) and P. gingivalis (10%). After rinsing the canal system using MTAD solution, there was a statistically significant decrease in E. faecalis (12%), P. intermedia (0%), T forsythensis (0%) and P. gingivalis (0%). The presence of other bacteria was also diminished but not statistically significantly.
Conclusion:
With the application of multiplex PCR technique which provided a simultaneous amplification of various genomic sequences, using several pairs of primers, the most dominant in infected root canals were E. faecalis. P. intermedia, A. actinomycetemcomitans, T. denticola, T. forsythensis and P. gingivalis. After mechanic treatment and irrigation of root canals with MTAD solution, P. intermedia, P. gingivalis and T forsythensis were not found. The presence of E. faecalis, A. actinomycetemcomitans and T. denticola was diminished, however, not statistically significantly.
Insights
The study found that MTAD solution significantly reduced key bacteria like Enterococcus faecalis and Prevotella intermedia in infected root canals. This dental antiseptic shows promise for treating apical periodontitis.
Area of Science:
- Microbiology
- Endodontics
- Molecular Biology
Context:
- Apical periodontitis is a common dental issue requiring effective antimicrobial strategies.
- Clinically acceptable antiseptics must balance efficacy with safety.
- Identifying microbial profiles in infected root canals is crucial for treatment planning.
Purpose:
- To assess the efficacy of MTAD (a solution of doxycycline, citric acid, and Tween-80) in reducing specific bacterial genomes in root canals.
- To quantify the presence of key pathogens including Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, and Enterococcus faecalis before and after MTAD treatment.
- To evaluate the impact of MTAD irrigation on the microbial load in primary endodontic infections.
Summary:
- Multiplex PCR detected a high prevalence of bacteria in infected root canals, with Enterococcus faecalis being the most dominant.
- Following irrigation with MTAD solution, a statistically significant reduction was observed for Enterococcus faecalis, Prevotella intermedia, Tanerella forsythensis, and Porphyromonas gingivalis.
- While the presence of Aggregatibacter actinomycetemcomitans and Treponema denticola also decreased, the reduction was not statistically significant.
Impact:
- MTAD demonstrates significant antimicrobial activity against several key bacteria implicated in apical periodontitis.
- The findings support the use of MTAD as an effective irrigant in endodontic treatment, potentially improving outcomes.
- This research contributes to understanding the microbial dynamics in root canal infections and the effectiveness of specific irrigating solutions.
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