Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Two-Dimensional (2D) NMR: Overview01:12

Two-Dimensional (2D) NMR: Overview

The 1D NMR spectrum of large and complex molecules like natural products has complicated splitting patterns and overlapping signals, which can be easily interpreted using 2-dimensional (2D) NMR. Unlike 1D NMR, 2D NMR has two frequency axes that provide the coupling information between the nucleus A and nucleus B in a molecule. The process from which 2D spectra are obtained has four steps.
The first step is the preparation period, during which nucleus A is excited with a radiofrequency pulse.
¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are slanted or...
2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)01:19

2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...
NMR Spectrometers: Overview01:20

NMR Spectrometers: Overview

NMR spectrometers consist of a strong magnet, a radiofrequency transmitter, and a detector attached to a computer console for recording spectra of samples containing NMR-active nuclei. In first-generation NMR instruments called continuous-wave spectrometers, the resonance frequencies of the nuclei are determined by frequency-sweep or field-sweep methods. The magnetic field strength is fixed and the rf signal is swept in the former, while the radiofrequency signal is fixed and the magnetic field...
2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
NMR Spectroscopy: Spin–Spin Coupling01:08

NMR Spectroscopy: Spin–Spin Coupling

The spin state of an NMR-active nucleus can have a slight effect on its immediate electronic environment. This effect propagates through the intervening bonds and affects the electronic environments of NMR-active nuclei up to three bonds away; occasionally, even farther. This phenomenon is called spin–spin coupling or J-coupling. Coupling interactions are mutual and result in small changes in the absorption frequencies of both nuclei involved. While nuclei of the same element are involved in...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Enhanced solid-state NMR sensitivity of α-synuclein fibrils using a MAS cryoprobe.

Biophysical chemistry·2026
Same author

Nuclear hyperfine interactions in critical metal AlB<sub>2</sub>-structured diborides and correlations to physical properties.

Solid state nuclear magnetic resonance·2026
Same author

Multinuclear Solid-State NMR and NMR Crystallography of Solid Forms of Creatine and Creatinine.

Molecular pharmaceutics·2026
Same author

Broadband cross polarization for ultra-wideline magic-angle spinning NMR.

Physical chemistry chemical physics : PCCP·2026
Same author

Effects of Structure and Bonding on <sup>195</sup>Pt Magnetic Shielding Tensors: Insights from Relativistic DFT and Localized Molecular Orbital Analysis.

Inorganic chemistry·2026
Same author

Impact of shared facilities in advancing solid-state NMR research: 2025 edition.

Solid state nuclear magnetic resonance·2025

Related Experiment Video

Updated: May 10, 2026

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
14:55

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Published on: September 17, 2017

Ultra-wideline solid-state NMR spectroscopy.

Robert W Schurko1

  • 1Department of Chemistry and Biochemistry, University of Windsor , Windsor, ON, Canada N9B 3P4.

Accounts of Chemical Research
|June 11, 2013
PubMed
Summary

Solid-state NMR (SSNMR) is often insensitive, but new ultra-wideline NMR (UWNMR) methods improve signal acquisition for challenging nuclides. These techniques enable the study of previously unobservable nuclei, expanding SSNMR

Area of Science:

  • Solid-state NMR Spectroscopy
  • Materials Science
  • Physical Chemistry

Background:

  • Solid-state NMR (SSNMR) is a powerful technique for analyzing molecular structure and dynamics.
  • However, SSNMR suffers from low sensitivity due to small spin population differences.
  • Factors like low gyromagnetic ratios, low natural abundance, and broad spectral patterns further challenge signal detection.

Purpose of the Study:

  • To describe recent advancements in pulse sequences and strategies for efficient acquisition of ultra-wideline NMR (UWNMR) spectra.
  • To introduce methodologies for overcoming the limitations of conventional SSNMR for challenging nuclides.
  • To highlight the application of these new UWNMR methods.

Main Methods:

  • Development of specialized pulse sequences for broadband excitation and refocusing.

More Related Videos

High-Resolution Neutron Spectroscopy to Study Picosecond-Nanosecond Dynamics of Proteins and Hydration Water
08:48

High-Resolution Neutron Spectroscopy to Study Picosecond-Nanosecond Dynamics of Proteins and Hydration Water

Published on: April 28, 2022

Related Experiment Videos

Last Updated: May 10, 2026

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
14:55

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Published on: September 17, 2017

High-Resolution Neutron Spectroscopy to Study Picosecond-Nanosecond Dynamics of Proteins and Hydration Water
08:48

High-Resolution Neutron Spectroscopy to Study Picosecond-Nanosecond Dynamics of Proteins and Hydration Water

Published on: April 28, 2022

  • Application of Carr-Purcell-Meiboom-Gill (CPMG) echo trains for signal acquisition.
  • Utilizing modern Fourier Transform (FT) NMR hardware and innovative acquisition strategies.

    • Main Results:

    • Demonstration of efficient acquisition of broad NMR powder patterns for various spin-1/2 and quadrupolar nuclides.
    • Successful application of UWNMR methods to previously unobservable or unreceptive NMR-active nuclides.
    • Acquisition of high-quality spectra for samples with spectral breadths ranging from 250 kHz to tens of MHz.

    Conclusions:

    • Ultra-wideline NMR (UWNMR) spectroscopy represents a significant advancement in SSNMR sensitivity and applicability.
    • These new methodologies enable the routine examination of nuclides that were previously considered too challenging for SSNMR.
    • UWNMR opens new avenues for studying diverse materials and biological systems with unprecedented detail.