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Updated: May 10, 2026
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Generation of Plasmid Vectors Expressing FLAG-tagged Proteins Under the Regulation of Human Elongation Factor-1α Promoter Using Gibson Assembly
Published on: February 9, 2015
[Construction and biological function of eukaryotic expression vector for myc-tagged HER2]
Jinjie Song1, Tao Wang, Xiaojie Xu
1Medical School of Chinese PLA, Beijing 100048, China. songjinjie520@sina.com
Summary
This study successfully created a myc-tagged human HER2 expression vector. This tool helps investigate HER2's role in breast cancer cell growth and trastuzumab drug resistance.
Area of Science:
- Molecular biology
- Oncology
- Biotechnology
Context:
- Human Epidermal growth factor Receptor 2 (HER2) is a key oncogene implicated in aggressive breast cancer.
- Understanding HER2's role in proliferation and drug resistance is crucial for targeted therapy development.
Purpose:
- To construct a eukaryotic expression vector for myc-tagged human HER2.
- To investigate the functional impact of myc-HER2 expression on breast cancer cell proliferation.
- To assess the sensitivity of cells overexpressing myc-HER2 to trastuzumab treatment.
Summary:
- A myc-HER2 eukaryotic expression vector was successfully constructed and validated through enzyme digestion and sequencing.
- Transfection of the myc-HER2 vector into ZR75-1 breast cancer cells led to significantly increased cell proliferation.
- Cells overexpressing myc-HER2 exhibited sensitivity to trastuzumab, an anti-HER2 antibody, indicating its potential role in drug resistance.
Impact:
- Provides a foundational tool for further research into HER2-mediated breast cancer progression.
- Offers insights into the mechanisms underlying HER2-driven cell proliferation and trastuzumab resistance.
- Facilitates the development of novel therapeutic strategies targeting HER2-positive breast cancers.
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