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The D box meets its match.

David O Morgan1

  • 1Department of Physiology, University of California, San Francisco, San Francisco, CA 94158, USA. david.morgan@ucsf.edu

Molecular Cell
|June 11, 2013
PubMed
Summary
This summary is machine-generated.

Researchers revealed the high-resolution structure of the destruction box bound to the anaphase-promoting complex, a key regulator in cell-cycle control. This finding advances our understanding of cell division mechanisms.

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Area of Science:

  • Molecular Biology
  • Cell Biology
  • Structural Biology

Background:

  • The cell cycle is a tightly regulated process essential for cell division.
  • The anaphase-promoting complex (APC/C) is a crucial ubiquitin ligase that controls cell cycle progression.
  • Specific sequences, like the destruction box (D-box), target proteins for degradation by the APC/C.

Purpose of the Study:

  • To elucidate the structural basis of the interaction between the destruction box motif and the anaphase-promoting complex.
  • To provide a high-resolution structural insight into a key regulatory interface of cell-cycle control.

Main Methods:

  • X-ray crystallography was employed to determine the structure.
  • Biochemical assays were used to validate the interaction.

Main Results:

  • A high-resolution structure of the destruction box sequence bound to the anaphase-promoting complex was determined.
  • The structure reveals the precise atomic interactions mediating substrate recognition and binding by the APC/C.

Conclusions:

  • The unveiled structure provides critical insights into the mechanism of APC/C-mediated protein degradation.
  • This structural information can inform the development of novel therapeutics targeting cell-cycle dysregulation.