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Predicting lymph node metastasis in early colorectal cancer using the CITED1 expression
Toru Nasu1, Yoshimasa Oku, Katsunari Takifuji
1Second Department of Surgery, Wakayama Medical University, School of Medicine, Wakayama, Japan.
The Journal of Surgical Research
|June 11, 2013
Summary
The gene CITED1 is a potential predictor of lymph node metastasis in colorectal cancer (CRC). High CITED1 expression in T1 CRC patients indicates a higher risk of metastasis, aiding treatment decisions.
Area of Science:
- Oncology
- Molecular Biology
- Cancer Biomarkers
Background:
- Accurate prediction of lymph node metastasis is crucial for T1 colorectal cancer (CRC) treatment strategies.
- Previous gene expression analysis identified 10 candidate genes associated with CRC de-differentiation at the invasion front.
- These genes show potential as markers for predicting lymph node metastasis in CRC.
Purpose of the Study:
- To identify molecular markers for predicting lymph node metastasis in colorectal cancer (CRC).
- To evaluate the potential of CITED1 as a predictive marker for lymph node metastasis in T1 CRC.
Main Methods:
- Quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) on 66 T3 CRC samples.
- Immunohistochemical analysis of CITED1 expression in 66 T3 and 95 T1 CRC samples.
- Univariate and multivariate logistic regression models to identify independent risk factors for lymph node metastasis.
Main Results:
- CITED1 messenger RNA expression was an independent risk factor for lymph node metastasis in T3 CRC (P=0.040).
- High CITED1 protein expression independently predicted lymph node metastasis in both T3 (P=0.035) and T1 CRC (P=0.010) patients.
- In T1 CRC, CITED1 protein expression had a negative predictive value of 95.5% for lymph node metastasis.
Conclusions:
- CITED1 expression correlates with lymph node metastasis in colorectal cancer patients.
- CITED1 protein expression demonstrates potential as a predictive biomarker for lymph node metastasis in T1 CRC, informing clinical management.
