Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
Neurogenesis and Regeneration of Nervous Tissue01:15

Neurogenesis and Regeneration of Nervous Tissue

In the CNS, neurogenesis, the birth of new neurons from stem cells, is limited to the hippocampus in adults. In other regions of the brain and spinal cord, neurogenesis is almost non-existent due to inhibitory influences from neuroglia, especially oligodendrocytes, and the absence of growth-stimulating cues. The myelin produced by oligodendrocytes in the CNS inhibits neuronal regeneration. Furthermore, astrocytes proliferate rapidly after neuronal damage, forming scar tissue that physically...
Synaptic Signaling01:09

Synaptic Signaling

Neurons communicate at synapses, or junctions, to excite or inhibit the activity of other neurons or target cells, such as muscles. Synapses may be chemical or electrical.
Most synapses are chemical, meaning an electrical impulse or action potential spurs the release of chemical messengers called neurotransmitters. The neuron sending the signal is called the presynaptic neuron, and the neuron receiving the signal is the postsynaptic neuron.
The presynaptic neuron fires an action potential that...
Synaptic Signaling01:12

Synaptic Signaling

Neurons communicate at synapses, or junctions, to excite or inhibit the activity of other neurons or target cells, such as muscles. Synapses may be chemical or electrical.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effects of Intravenous Lidocaine on Rheumatoid Arthritis in Preclinical Rat Model and Clinical Trial.

Journal of pain research·2026
Same author

Elimination of tau tangles and soluble aggregates with the small molecule ACI-16664 prevents neurodegeneration in vivo.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2026
Same author

The role of prostanoids in the retina and optic nerve in health and disease.

Progress in retinal and eye research·2026
Same author

Lipidomic analysis reveals drug-induced lipoxin synthesis in glaucoma treatment.

JCI insight·2026
Same author

Lipoxin B4 Mitigates TRPV4-Activated Müller Cell Gliosis During Ocular Hypertension.

Investigative ophthalmology & visual science·2026
Same author

Association between obstructive sleep apnea and the risk of cerebrovascular disease in COPD patients.

Frontiers in medicine·2026

Related Experiment Video

Updated: May 10, 2026

Morphological and Functional Evaluation of Axons and their Synapses during Axon Death in Drosophila melanogaster
10:29

Morphological and Functional Evaluation of Axons and their Synapses during Axon Death in Drosophila melanogaster

Published on: March 16, 2020

This message will self-destruct: NMD regulates axon guidance.

Nicolas Preitner1, Jie Quan, John G Flanagan

  • 1Department of Cell Biology and Program in Neuroscience, Harvard Medical School, Boston, MA 02115, USA.

Cell
|June 11, 2013
PubMed
Summary

Regulated intra-axonal protein synthesis and nonsense-mediated mRNA decay (NMD) control Robo3.2 expression. This molecular switch is essential for guiding axons to their correct destinations during neural development.

More Related Videos

Standardization of a Novel Semi-Automatic Software for Neurite Outgrowth Measurement
05:28

Standardization of a Novel Semi-Automatic Software for Neurite Outgrowth Measurement

Published on: August 9, 2024

Assaying the Ability of Diffusible Signaling Molecules to Reorient Embryonic Spinal Commissural Axons
09:28

Assaying the Ability of Diffusible Signaling Molecules to Reorient Embryonic Spinal Commissural Axons

Published on: March 8, 2010

Related Experiment Videos

Last Updated: May 10, 2026

Morphological and Functional Evaluation of Axons and their Synapses during Axon Death in Drosophila melanogaster
10:29

Morphological and Functional Evaluation of Axons and their Synapses during Axon Death in Drosophila melanogaster

Published on: March 16, 2020

Standardization of a Novel Semi-Automatic Software for Neurite Outgrowth Measurement
05:28

Standardization of a Novel Semi-Automatic Software for Neurite Outgrowth Measurement

Published on: August 9, 2024

Assaying the Ability of Diffusible Signaling Molecules to Reorient Embryonic Spinal Commissural Axons
09:28

Assaying the Ability of Diffusible Signaling Molecules to Reorient Embryonic Spinal Commissural Axons

Published on: March 8, 2010

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Developmental Biology

Background:

  • Axon navigation is a complex process crucial for forming functional neural circuits.
  • Axon guidance relies on a series of molecular cues and receptor interactions.
  • Understanding the regulation of guidance receptors is key to deciphering neural development.

Purpose of the Study:

  • To investigate the role of intra-axonal protein synthesis in axon guidance.
  • To elucidate the mechanism controlling the expression of guidance receptors during navigation.
  • To identify the molecular players involved in regulating Robo3.2 function.

Main Methods:

  • Analysis of intra-axonal protein synthesis.
  • Investigation of nonsense-mediated mRNA decay (NMD) pathways.
  • Study of Robo3.2 expression dynamics.
  • Axon guidance assays in vivo and in vitro.

Main Results:

  • Demonstrated that regulated intra-axonal protein synthesis is essential for axon pathfinding.
  • Showcased the critical role of nonsense-mediated mRNA decay (NMD) in controlling Robo3.2 expression.
  • Identified a switch in Robo3.2 expression mediated by NMD, crucial for navigation.

Conclusions:

  • Intra-axonal protein synthesis coupled with NMD provides a regulatory mechanism for axon guidance.
  • This mechanism allows for dynamic changes in guidance receptor expression, enabling precise navigation.
  • The findings offer new insights into the molecular control of neural circuit formation.